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Invitrogen
Cortactin Polyclonal Antibody
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FIGURE: 1 / 10
Cortactin Antibody (PA5-27134) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Cortactin was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Cortactin Polyclonal Antibody (Product # PA5-27134) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-27134
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, HepG2, NIH-3T3, JC, PC-12, mouse brain, rat brain.
Predicted reactivity: Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cortactin (Cttn) is a ubiquitous actin-binding protein that was originally identified as a substrate for Src. It contributes to the organization of the actin cytoskeleton and cell shape. Cortactin also plays a role in the formation of lamellipodia and cell migration. It is accumulated in peripheral, actin-enriched structures of cells suggesting that cortactin facilitates actin network formation. Cortactin has four major domains of interest the N-terminal acidic (NTA) and tandem repeats domains and the C-terminal proline-rich and SH3 Domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin is involved in promoting cell motility and invasion, including a critical role in invadopodia, actin rich-subcellular protrusions associated with degradation of the ECM by cancer cells. Cortactin is phosphorylated by src family kinases at Y421, Y466, and Y482 and S405 and S418 that are phosphorylated by Erk family kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Amplaxin; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); epididymis secretory sperm binding protein; FLJ34459; mammary tumor and squamous cell carcinoma associated (p80/85 src substrate); Oncogene EMS1; Oncogene SRC8; Src substrate cortactin; src8; unnamed protein product
Gene Aliases: 1110020L01Rik; CTTN; Cttnb; EMS1
UniProt ID: (Human) Q14247, (Mouse) Q60598, (Rat) Q66HL2
Entrez Gene ID: (Human) 2017, (Mouse) 13043, (Rat) 60465
intracellular protein transport
endocytosis
receptor-mediated endocytosis
substrate-dependent cell migration, cell extension
signal transduction
actin cytoskeleton organization
regulation of axon extension
positive regulation of actin filament polymerization
positive regulation of smooth muscle contraction
focal adhesion assembly
neuron projection morphogenesis
cell motility
positive regulation of chemotaxis
regulation of cell projection assembly
dendritic spine maintenance
extrinsic apoptotic signaling pathway
lamellipodium organization
modification of postsynaptic actin cytoskeleton
modification of postsynaptic structure
regulation of mitophagy
negative regulation of extrinsic apoptotic signaling pathway
actin filament polymerization
actin cytoskeleton reorganization
regulation of mitophagy
brain development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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