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Invitrogen
PCNA Polyclonal Antibody
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FIGURE: 1 / 29
PCNA Antibody (PA5-27214) in ICC/IF
Immunofluorescence analysis of PCNA was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PCNA Polyclonal Antibody (Product # PA5-27214, 1:100 dilution) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:20... View More
Product Details
PA5-27214
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Hamster,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.43 mg/mL
Amount
43 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, PC-12, A549, SiHa.
Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (92%), Xenopus laevis (89%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CG9193-PA; CG9193-PB; Cyclin; DNA polymerase delta auxiliary protein; DNA sliding clamp PCNA; HGCN8729; LD45889p; MGC8367; mutagen-sensitive 209; Mutagen-sensitive 209 protein; PCNA; PCNA-PA; PCNA-PB; Proliferating cell nuclear antigen; proliferating-cell nuclear antigen; Proliferating-cell-nuclear-antigen; unnamed protein product
Gene Aliases: 53/13; ATLD2; CG9193; Dmel\CG9193; Dmel_CG9193; DmPCNA; DmPCNA1; dPCNA; l(2)02448; l(2)56F; l(2)56Fa; l(2)s1534; mus 209; MUS209; PCNA; Pcna/cyclin; PCNA/mus209; PCNA1; PCNAR
UniProt ID: (Fruit fly) P17917, (Human) P12004, (Rat) P04961, (Chinese hamster) P57761, (Mouse) P17918
Entrez Gene ID: (Fruit fly) 37290, (Human) 5111, (Rat) 25737, (Chinese hamster) 100689335, (Mouse) 18538
DNA binding
chromatin binding
DNA polymerase processivity factor activity
purine-specific mismatch base pair DNA N-glycosylase activity
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
macromolecular complex binding
DNA polymerase binding
transcription factor binding
DNA polymerase processivity factor
DNA replication
DNA-dependent DNA replication
leading strand elongation
regulation of DNA replication
nucleotide-excision repair
mismatch repair
cellular response to DNA damage stimulus
mitotic spindle organization
eggshell chorion gene amplification
response to UV
translesion synthesis
response to hydrogen peroxide
response to methyl methanesulfonate
negative regulation of transcription from RNA polymerase II promoter
DNA repair
base-excision repair, gap-filling
chromatin organization
response to oxidative stress
heart development
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
response to estradiol
response to lipid
cellular response to UV
estrous cycle
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
cellular response to hydrogen peroxide
cellular response to xenobiotic stimulus
response to dexamethasone
liver regeneration
positive regulation of DNA-directed DNA polymerase activity
response to L-glutamate
mitotic telomere maintenance via semi-conservative replication
regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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