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          • Primary Antibodies ›
          • CRK Antibodies

          Invitrogen

          Crk Polyclonal Antibody

          View all (25) CRK antibodies

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          Cite Crk Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          Crk Antibody in Immunocytochemistry (ICC/IF)
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          Crk Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Crk Antibody (PA5-27293) in ICC/IF

          Immunocytochemistry analysis of Crk in methanol-fixed A431. Samples were incubated with Crk polyclonal antibody (Product # PA5-27293) using a dilution of 1:200. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Crk Antibody in Immunocytochemistry (ICC/IF)
          Crk Antibody in Western Blot (WB)
          Crk Antibody in Western Blot (WB)
          Crk Antibody in Western Blot (WB)

          Product Details

          PA5-27293

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 40% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2544769

          Product Specific Information

          Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3.

          Predicted reactivity: Mouse (100%), Rat (99%), Xenopus laevis (89%), Pig (98%), Chicken (91%), Bovine (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; CRK1; CSAID-binding protein; CT-10 related kinase 3; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; encoded by transcript variant 4; p38 MAP kinase; encoded by transcript variant 5; p38 MAP kinase; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; p38; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; Proto-oncogene c-Crk; SAPK2a; SAPK2a; CSAIDS binding protein 1; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38; unnamed protein product; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog (avian) isoform CRK_1; v-crk sarcoma virus CT10 oncogene homolog (avian) isoform CRK_2; v-crk sarcoma virus CT10 oncogene-like protein

          View more View less

          Gene Aliases: c-Crk; CRK; Crk-I; Crk-II; Crk-III; Crk1; Crk3; CRKII; CrkIII; Crko; CSBP; CSBP1; CSBP2; CSPB1; EXIP; MAPK14; MXI2; p38; p38-alpha; p38a; p38ALPHA; p38MAPK; PRKM14; PRKM15; RK; SAPK2A

          View more View less

          UniProt ID: (Human) P46108, (Mouse) Q64010, (Human) Q16539, (Mouse) P47811

          View more View less

          Entrez Gene ID: (Human) 1398, (Mouse) 12928, (Human) 1432, (Mouse) 26416

          View more View less

          Function(s)
          phosphotyrosine binding receptor binding insulin-like growth factor receptor binding protein binding cytoskeletal protein binding SH3 domain binding enzyme binding kinase binding protein domain specific binding receptor signaling complex scaffold activity protein binding, bridging receptor tyrosine kinase binding ubiquitin protein ligase binding signaling adaptor activity SH2 domain binding protein phosphorylated amino acid binding ephrin receptor binding scaffold protein binding protein tyrosine kinase binding SH3/SH2 adaptor activity nucleotide binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity MAP kinase kinase activity ATP binding kinase activity transferase activity protein phosphatase binding mitogen-activated protein kinase p38 binding NFAT protein binding protein serine kinase activity protein C-terminus binding transferase activity, transferring phosphorus-containing groups scaffold/adaptor protein non-receptor serine/threonine protein kinase
          Process(es)
          neuron migration response to yeast regulation of leukocyte migration regulation of transcription from RNA polymerase II promoter lipid metabolic process enzyme linked receptor protein signaling pathway cell proliferation regulation of cell shape regulation of signal transduction positive regulation of smooth muscle cell migration dendrite development cell migration hippocampus development cerebral cortex development establishment of cell polarity actin cytoskeleton organization positive regulation of cell growth regulation of actin cytoskeleton organization regulation of cell adhesion mediated by integrin regulation of Rac protein signal transduction positive regulation of Rac protein signal transduction helper T cell diapedesis response to hepatocyte growth factor reelin-mediated signaling pathway response to hydrogen peroxide regulation of GTPase activity negative regulation of natural killer cell mediated cytotoxicity positive regulation of JNK cascade ephrin receptor signaling pathway regulation of dendrite development cell chemotaxis regulation of wound healing negative regulation of wound healing response to cholecystokinin cellular response to transforming growth factor beta stimulus cellular response to nitric oxide protein localization to membrane postsynaptic specialization assembly cerebellar neuron development positive regulation of substrate adhesion-dependent cell spreading response to peptide regulation of intracellular signal transduction positive regulation of skeletal muscle acetylcholine-gated channel clustering cellular response to nerve growth factor stimulus cellular response to insulin-like growth factor stimulus cellular response to endothelin regulation of cell motility negative regulation of cell motility regulation of T cell migration positive regulation of signal transduction DNA damage checkpoint MAPK cascade cell morphogenesis cartilage condensation angiogenesis osteoblast differentiation placenta development response to dietary excess chondrocyte differentiation negative regulation of inflammatory response to antigenic stimulus glucose metabolic process regulation of transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process chemotaxis cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway transmembrane receptor protein serine/threonine kinase signaling pathway skeletal muscle tissue development positive regulation of gene expression positive regulation of myotube differentiation fatty acid oxidation platelet activation regulation of ossification osteoclast differentiation stress-activated protein kinase signaling cascade positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway response to muramyl dipeptide response to lipopolysaccharide positive regulation of interleukin-12 production response to insulin tumor necrosis factor-mediated signaling pathway negative regulation of hippo signaling intracellular signal transduction cellular response to vascular endothelial growth factor stimulus response to muscle stretch p38MAPK cascade positive regulation of protein import into nucleus signal transduction in response to DNA damage positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter glucose import positive regulation of glucose import vascular endothelial growth factor receptor signaling pathway stem cell differentiation striated muscle cell differentiation positive regulation of muscle cell differentiation stress-activated MAPK cascade protein maturation positive regulation of cardiac muscle cell proliferation bone development pyroptosis 3'-UTR-mediated mRNA stabilization cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to tumor necrosis factor cellular response to ionizing radiation cellular response to UV-B negative regulation of canonical Wnt signaling pathway positive regulation of brown fat cell differentiation cellular senescence stress-induced premature senescence cellular response to virus regulation of synaptic membrane adhesion regulation of cytokine production involved in inflammatory response positive regulation of myoblast fusion NLRP1 inflammasome complex assembly positive regulation of reactive oxygen species metabolic process transcription, DNA-templated protein phosphorylation myoblast differentiation involved in skeletal muscle regeneration phosphorylation peptidyl-serine phosphorylation protein autophosphorylation
          It has to be done as per old AB suggested Products section.

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