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Invitrogen
OCRL Polyclonal Antibody
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FIGURE: 1 / 3
OCRL Antibody (PA5-27844) in ICC/IF
OCRL antibody detects OCRL protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: OCRL protein stained by OCRL antibody (Product # PA5-27844) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-27844
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human INPP5F. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431.
Predicted reactivity: Mouse (95%), Rat (96%), Rhesus Monkey (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The inositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of various phosphatidylinositols, which generate second messengers in response to extracellular signals. OCRL1 is a type II 5-phosphatase that is mutated in the oculocerebrorenal syndrome of Lowe (OCRL). OCRL is a rare X-linked disorder that is characterized in part by congenital cataracts, mental retardation, muscular hypotonia and renal tubular dysfunction. OCRL1 has a high affinity for phosphatidylinositol 4,5-bisphosphate as well as inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate as substrates. OCRL1 is localized to the Golgi complex and is thought to be part of the trans-Golgi network (TGN), which suggests that OCRL1 plays a role in protein sorting and trafficking within the cell.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Inositol polyphosphate 5-phosphatase OCRL; Inositol polyphosphate 5-phosphatase OCRL-1; Lowe oculocerebrorenal syndrome protein; OCRL-1; oculocerebrorenal syndrome of Lowe; Phosphatidylinositol 3,4,5-triphosphate 5-phosphatase; phosphatidylinositol polyphosphate 5-phosphatase; Unknown; unnamed protein product
Gene Aliases: Dent-2; DENT2; INPP5F; LOCR; NPHL2; OCRL; OCRL-1; OCRL1
UniProt ID: (Human) Q01968
Entrez Gene ID: (Human) 4952
phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
inositol-polyphosphate 5-phosphatase activity
GTPase activator activity
protein binding
hydrolase activity
phosphatase activity
small GTPase binding
phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity
phosphatidylinositol bisphosphate phosphatase activity
phosphatidylinositol phosphate 5-phosphatase activity
phosphatidylinositol phosphate 4-phosphatase activity
phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity
inositol-1,4,5-trisphosphate 5-phosphatase activity
inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity
inositol phosphate phosphatase activity
in utero embryonic development
lipid metabolic process
phosphatidylinositol biosynthetic process
signal transduction
cell projection organization
phosphatidylinositol-3-phosphate biosynthetic process
inositol phosphate metabolic process
phosphatidylinositol metabolic process
phosphatidylinositol dephosphorylation
cilium morphogenesis
membrane organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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