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Invitrogen
KLF5 Polyclonal Antibody
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FIGURE: 1 / 8
KLF5 Antibody (PA5-27876) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of KLF5 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Product Details
PA5-27876
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A549, HCT116, KLF5-transfected 293T.
Predicted reactivity: Mouse (89%), Rat (90%), Pig (96%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. It contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. KLF5 is a transcription activator that promotes cell proliferation and oncogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: (intestinal Kruppel-like factor; basic transcription element binding protein 2; basic transcription element binding protein BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; epididymis secretory sperm binding protein; GC box binding protein 2; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Krueppel-like factor 5; Kruppel like factor 5; Kruppel-like factor 5 (intestinal); RP11-505F3.5; testis-specific transcript; tKLF5; transcription factor; Transcription factor BTEB2; unnamed protein product
Gene Aliases: 4930520J07Rik; BTEB2; CKLF; IKLF; KLF5
UniProt ID: (Human) Q13887, (Mouse) Q9Z0Z7
Entrez Gene ID: (Human) 688, (Rat) 84410, (Mouse) 12224
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
MRF binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
regulation of transcription from RNA polymerase II promoter
positive regulation of cell proliferation
regulation of gene expression
skeletal muscle satellite cell differentiation
satellite cell activation involved in skeletal muscle regeneration
myotube differentiation involved in skeletal muscle regeneration
microvillus assembly
regulation of microvillus assembly
skeletal muscle cell differentiation
skeletal muscle tissue regeneration
positive regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
intestinal epithelial cell development
positive regulation of transcription by transcription factor localization
cellular response to leukemia inhibitory factor
cellular response to endothelin
cellular response to organic cyclic compound
cellular response to peptide
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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