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Invitrogen
CDK6 Polyclonal Antibody
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FIGURE: 1 / 11
CDK6 Antibody (PA5-27978) in ICC/IF
Immunofluorescence analysis of CDK6 was performed using 70% confluent log phase MOLT-4 and MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with CDK6 Polyclonal Antibody (Product # PA5-27978) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (... View More
Product Details
PA5-27978
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human CDK6. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Amount
70 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (85%), Chicken (93%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CDK6 functions as a cell-cycle initiator protein. This protein is coexpressed and copurified with CyclinD1. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cell division protein kinase 6; CR2 protein kinase; CRK2; Cyclin-dependent kinase 6; MGC59692; Serine/threonine-protein kinase PLSTIRE; unnamed protein product
Gene Aliases: 5830411I20; AI504062; CDK6; CDKN6; Crk2; MCPH12; PLSTIRE
UniProt ID: (Human) Q00534, (Mouse) Q64261
Entrez Gene ID: (Human) 1021, (Rat) 114483, (Mouse) 12571
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
cyclin binding
FBXO family protein binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cell-matrix adhesion
hematopoietic progenitor cell differentiation
type B pancreatic cell development
cellular response to DNA damage stimulus
signal transduction
Notch signaling pathway
negative regulation of cell proliferation
response to virus
regulation of G2/M transition of mitotic cell cycle
regulation of gene expression
positive regulation of gene expression
astrocyte development
dentate gyrus development
lateral ventricle development
hemopoiesis
cell differentiation
T cell differentiation in thymus
gliogenesis
regulation of cell proliferation
cell dedifferentiation
negative regulation of cell differentiation
negative regulation of myeloid cell differentiation
regulation of erythrocyte differentiation
negative regulation of monocyte differentiation
negative regulation of osteoblast differentiation
negative regulation of cell cycle
positive regulation of fibroblast proliferation
generation of neurons
negative regulation of epithelial cell proliferation
cell division
regulation of cell cycle
hematopoietic stem cell differentiation
regulation of hematopoietic stem cell differentiation
regulation of cell motility
negative regulation of cellular senescence
protein phosphorylation
phosphorylation
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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