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Invitrogen
TRIF Polyclonal Antibody
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FIGURE: 1 / 7
TRIF Antibody (PA5-28105) in ICC/IF
Immunofluorescence analysis of TIR domain-containing adapter molecule 1 was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TRIF Polyclonal Antibody (Product # PA5-28105) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Al... View More
Product Details
PA5-28105
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRIF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.01 mg/mL
Amount
101 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa.
Predicted reactivity: Rhesus Monkey (100%), Chimpanzee (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hCG_39321; HSD34; MyD88-3; Proline-rich, vinculin and TIR domain-containing protein B; Putative NF-kappa-B-activating protein 502H; RNF36; TI; TICAM-1; TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adapter protein inducing IFN-beta; toll like receptor adaptor molecule 1; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
Gene Aliases: IIAE6; MyD88-3; PRVTIRB; TICAM-1; TICAM1; TRIF
UniProt ID: (Human) Q8IUC6
Entrez Gene ID: (Human) 148022
toll-like receptor signaling pathway
macrophage activation involved in immune response
immune system process
positive regulation of myeloid dendritic cell cytokine production
MyD88-independent toll-like receptor signaling pathway
nitric oxide biosynthetic process
apoptotic process
inflammatory response
signal transduction
cell surface receptor signaling pathway
positive regulation of autophagy
positive regulation of gene expression
positive regulation of B cell proliferation
positive regulation of protein ubiquitination
lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
response to lipopolysaccharide
positive regulation of chemokine production
positive regulation of interferon-beta production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell activation
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
TRIF-dependent toll-like receptor signaling pathway
B cell proliferation
B cell activation
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of protein complex assembly
response to exogenous dsRNA
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of B cell activation
defense response to virus
positive regulation of macrophage cytokine production
cellular response to lipopolysaccharide
apoptotic signaling pathway
cellular response to oxidised low-density lipoprotein particle stimulus
positive regulation of cytokine production involved in inflammatory response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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