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Invitrogen

MST2 Polyclonal Antibody

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Product Details

PA5-28567

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human STK3. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.84 mg/mL

Amount

84 µg

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546043

Product Specific Information

Recommended positive controls: 293T transfected STK3, , NIH-3T3, JC.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (98%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (99%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

STK3 (MST2) is a STE kinase family member that acts upstream of the MAP kinase signaling pathways, and is known to be activated by the proapoptotic stimuli staurosporine and FAS ligand. STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein; hippo homolog; KB-1458E12.1; Mammalian STE20-like protein kinase 2; MST-2; protein kinase homolog; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase Krs-1; STE20-like kinase MST2; unnamed protein product

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Gene Aliases: 0610042I06Rik; KRS1; Mess1; MST; MST2; Mst3; Ste20; STK3

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UniProt ID: (Human) Q13188, (Mouse) Q9JI10

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Entrez Gene ID: (Human) 6788, (Mouse) 56274

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Function(s)
nucleotide binding magnesium ion binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity transferase activity identical protein binding protein serine/threonine kinase activator activity metal ion binding protein serine kinase activity transcription regulator activator activity MAP kinase kinase kinase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
neural tube formation endocardium development protein phosphorylation protein import into nucleus apoptotic process signal transduction JNK cascade central nervous system development cell proliferation negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors regulation of gene expression organ growth hippo signaling positive regulation of hippo signaling intracellular signal transduction positive regulation of apoptotic process regulation of MAPK cascade protein kinase B signaling positive regulation of fat cell differentiation positive regulation of JNK cascade negative regulation of organ growth epithelial cell proliferation negative regulation of epithelial cell proliferation protein stabilization protein tetramerization centrosome separation positive regulation of protein kinase B signaling canonical Wnt signaling pathway primitive hemopoiesis cell differentiation involved in embryonic placenta development regulation of cell differentiation involved in embryonic placenta development protein localization to centrosome negative regulation of canonical Wnt signaling pathway hepatocyte apoptotic process positive regulation of extrinsic apoptotic signaling pathway via death domain receptors MAPK cascade activation of MAPKK activity regulation of mitotic cell cycle phosphorylation signal transduction by protein phosphorylation stress-activated protein kinase signaling cascade positive regulation of protein binding positive regulation of sequence-specific DNA binding transcription factor activity
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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