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Invitrogen
Histone H2A.X Polyclonal Antibody
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FIGURE: 1 / 19
Histone H2A.X Antibody (PA5-28778) in ICC/IF
Histone H2A. X Polyclonal Antibody detects Histone H2A. X protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A. X stained by Histone H2A. X Polyclonal Antibody (Product # PA5-28778) diluted at 1:2,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
Product Details
PA5-28778
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, HepG2, HCT116, HCT116 (30uM cisplatin for 24hr), Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, mouse brain, rat brain, PC-12, Rat2, 4T1.
Predicted reactivity: Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gamma H2AX; H2A histone family member X; H2A histone family, member X; H2a/x; H2AFX; H2AX histone; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX; unnamed protein product
Gene Aliases: AW228881; gammaH2ax; H2A.X; H2A/X; H2AFX; H2AX; Hist5-2ax; RGD1566119
UniProt ID: (Human) P16104, (Mouse) P27661
Entrez Gene ID: (Human) 3014, (Rat) 500987, (Mouse) 15270
DNA damage checkpoint
double-strand break repair via homologous recombination
DNA repair
double-strand break repair
DNA recombination
nucleosome assembly
cellular response to DNA damage stimulus
spermatogenesis
response to ionizing radiation
heterochromatin assembly
positive regulation of DNA repair
meiotic cell cycle
protein K63-linked ubiquitination
positive regulation of double-strand break repair via homologous recombination
protein localization to site of double-strand break
cerebral cortex development
cellular response to gamma radiation
cellular senescence
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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