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Invitrogen
MCM4 Polyclonal Antibody
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FIGURE: 1 / 10
MCM4 Antibody (PA5-29039) in ICC/IF
Immunofluorescence analysis of MCM4 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MCM4 Polyclonal Antibody (Product # PA5-29039) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Product Details
PA5-29039
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Jurkat, Raji, NCI-H929, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.
Predicted reactivity: Mouse (96%), Rat (95%), Zebrafish (88%), Xenopus laevis (89%), Rhesus Monkey (99%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC21 homolog; DNA replication licensing factor MCM4; homolog of S. pombe cell devision cycle 21; MCM4 minichromosome maintenance deficient 4, mitotin; mini chromosome maintenance deficient 4 homolog; minichromosome maintenance deficient 4; P1-CDC21; unnamed protein product
Gene Aliases: 19G; AI325074; AU045576; cb1025; CDC21; CDC54; fc12c09; fj85g09; hCdc21; hm:zeh1616; IMD54; mcdc21; MCM4; Mcmd4; mKIAA4003; NKCD; NKGCD; P1-CDC21; wu:fc12c09; wu:fj85g09; zeh1616
UniProt ID: (Human) P33991, (Mouse) P49717
Entrez Gene ID: (Human) 4173, (Mouse) 17217, (Zebrafish) 337598
nucleotide binding
DNA binding
DNA helicase activity
single-stranded DNA binding
helicase activity
protein binding
ATP binding
hydrolase activity
ATPase activity
single-stranded DNA-dependent ATP-dependent DNA helicase activity
ATP-dependent DNA helicase activity
chromatin binding
DNA replication origin binding
DNA metabolism protein
double-strand break repair via break-induced replication
DNA replication
DNA replication initiation
DNA strand elongation involved in DNA replication
regulation of DNA-dependent DNA replication initiation
mitotic DNA replication initiation
DNA unwinding involved in DNA replication
cell cycle
response to yeast
pre-replicative complex assembly involved in nuclear cell cycle DNA replication
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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