Search
Invitrogen
SMC3 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 6
SMC3 Antibody (PA5-29052) in WB
Western blot was performed using Anti-SMC3 Polyclonal Antibody (Product # PA5-29052) and a 160 kDa band corresponding to SMC3 was observed in all the tested cell and tissue models. Modified whole cell lysate (1% SDS) (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), A549 (Lane 4), U-87 MG (Lane 5), SH-SY5Y (Lane 6), HCT 116 (Lane 7), Jurkat (Lane 8) and tissue extract (30 µg lysate) of Mouse Liver (Lane 9) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto ... View More
Product Details
PA5-29052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SMC3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.95 mg/mL
Amount
95 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse liver.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (98%), Xenopus laevis (99%), Chicken (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Bamacan; Basement membrane-associated chondroitin proteoglycan; CG9802-PA; Chondroitin sulfate proteoglycan 6; chondroitin sulfate proteoglycan 6 (bamacan); Chromosome segregation protein SmcD; Chromosome-associated polypeptide; Chromosome-associated protein; cohesin subunit SMC3; hCAP; Mad member-interacting protein 1; SMC family; SMC protein 3; SMC3-PA; structural maintenace of chromosomes 3; Structural maintenance of chromosomes protein 3; unnamed protein product
Gene Aliases: BAM; Bamacan; BMH; CAP; CDLS3; CG9802; CSPG6; dCAP; Dmel\CG9802; Dmel_CG9802; DmSMC3; DmSMC3/Cap; dSMC3; HCAP; im:7142991; Mmip1; SMC-3; SMC3; SMC3L1; SmcD; wu:fb22e01; wu:fc30d07
UniProt ID: (Human) Q9UQE7, (Mouse) Q9CW03
Entrez Gene ID: (Fruit fly) 32627, (Human) 9126, (Mouse) 13006, (Zebrafish) 324475
chromatin binding
ATP binding
protein heterodimerization activity
nucleotide binding
core promoter proximal region sequence-specific DNA binding
double-stranded DNA binding
microtubule motor activity
protein binding
ATPase activity
mediator complex binding
beta-tubulin binding
dynein complex binding
dynein binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
regulation of DNA replication
DNA repair
cellular response to DNA damage stimulus
cell cycle
sister chromatid cohesion
mitotic sister chromatid cohesion
mitotic nuclear division
meiotic nuclear division
signal transduction
stem cell population maintenance
negative regulation of DNA endoreduplication
chromosome organization
cell division
meiotic cell cycle
establishment of imaginal disc-derived wing hair orientation
imaginal disc-derived wing morphogenesis
mitotic cell cycle
establishment of mitotic sister chromatid cohesion
establishment of meiotic sister chromatid cohesion
mitotic spindle assembly
regulation of transcription from RNA polymerase II promoter
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support