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Invitrogen
ATP Citrate Lyase Polyclonal Antibody
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FIGURE: 1 / 8
ATP Citrate Lyase Antibody (PA5-29497) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ATP Citrate Lyase was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: ATP Citrate Lyase Polyclonal Antibody (Product # PA5 29497) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-29497
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.93 mg/mL
Amount
93 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain, rat brain.
Predicted reactivity: Mouse (96%), Rat (96%), Pig (95%), Chicken (85%), Sheep (97%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme; unnamed protein product
Gene Aliases: A730098H14Rik; ACL; ACLY; ATPCL; AW538652; CLATP
UniProt ID: (Human) P53396, (Mouse) Q91V92, (Rat) P16638
Entrez Gene ID: (Human) 47, (Mouse) 104112, (Rat) 24159
nucleotide binding
catalytic activity
ATP citrate synthase activity
protein binding
ATP binding
transferase activity
metal ion binding
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
succinate-CoA ligase (ADP-forming) activity
cofactor binding
lyase activity
transferase
acetyl-CoA biosynthetic process
citrate metabolic process
oxaloacetate metabolic process
lipid metabolic process
fatty acid biosynthetic process
cholesterol biosynthetic process
lipid biosynthetic process
coenzyme A metabolic process
negative regulation of ferroptosis
acetyl-CoA metabolic process
metabolic process
cellular carbohydrate metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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