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Invitrogen
PBX1 Polyclonal Antibody
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FIGURE: 1 / 9
PBX1 Antibody (PA5-29674) in ICC/IF
Immunofluorescence analysis of PBX1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PBX1 Polyclonal Antibody (Product # PA5-29674) at 1:1000 dilution, in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More
Product Details
PA5-29674
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PBX1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A431.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Chicken (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1; 19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686B09108; Homeobox protein PBX1; Homeobox protein PRL; homeobox-containing protein; MGC126627; OTTHUMP00000032205; pre-B-cell leukemia homeobox 1; Pre-B-cell leukemia transcription factor 1; unnamed protein product
Gene Aliases: CAKUHED; PBX1; PRL
UniProt ID: (Human) P40424
Entrez Gene ID: (Human) 5087
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcription corepressor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
G2/M transition of mitotic cell cycle
eye development
urogenital system development
branching involved in ureteric bud morphogenesis
natural killer cell differentiation
regulation of transcription, DNA-templated
steroid biosynthetic process
brain development
sex differentiation
cell proliferation
organ morphogenesis
anterior/posterior pattern specification
proximal/distal pattern formation
positive regulation of G2/M transition of mitotic cell cycle
cell differentiation
regulation of ossification
adrenal gland development
embryonic limb morphogenesis
embryonic hemopoiesis
regulation of cell proliferation
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
spleen development
thymus development
embryonic organ development
neuron development
embryonic skeletal system development
stem cell proliferation
positive regulation of stem cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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