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Invitrogen
LC3B Polyclonal Antibody
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FIGURE: 1 / 19
LC3B Antibody (PA5-32254) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of LC3B in HeLa cells mock (left) and in HeLa cells treated with 50 µM Chloroquine for 24 hr (right) fixed with 4% paraformaldehyde at RT for 15 min. Green: LC3B Polyclonal Antibody (Product # PA5-32254) diluted at 1:2,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.
Product Details
PA5-32254
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Insect,
Mouse,
Pig,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, mouse brain, Rat brain, HepG2 (untreated), HepG2 (3 µM Thapsigargin treatment for 12 hr), HepG2 (3 µM Thapsigargin treatment for 16hr), HepG2 (3 µM Thapsigargin treatment for 24hr), Huh7 (un-infected), Huh7 (HCV-infected), HeLa, HeLa (50 µM Chloroquine treatment for 24 hr).
Predicted reactivity: Zebrafish (100%), Japanese Medaka (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATG8E; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3 beta; LC3A; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1BLC3; MAP1LC3A; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; RP11-346K17.1
Gene Aliases: 1010001C15Rik; Atg8; ATG8F; ATG8G; LC3B; MAP1A/1BLC3; MAP1ALC3; Map1lc3; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; zbs559
UniProt ID: (Human) Q9GZQ8, (Human) A6NCE7, (Rat) Q62625, (Mouse) Q9CQV6
Entrez Gene ID: (Human) 81631, (Pig) 100462754, (Human) 643246, (Rat) 64862, (Mouse) 67443
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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