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Invitrogen
Phospho-HEC1 (Ser165) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-HEC1 (Ser165) Antibody (PA5-34978) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Phospho-HEC1-(Ser165) in MCF10A cells using Phospho-HEC1 (Ser165) Polyclonal Antibody (Product # PA5-34978). Insects show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
Product Details
PA5-34978
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rodent,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to the amino acids around Ser 165 conjugated to KLH.
Conjugate
Form
Liquid
Concentration
0.127 mg/mL
Amount
12.7 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, Blocking peptide (GTX70013-PEP).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Hec1 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HEC-1; HEC2; HEC4; Highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats; HsHec1; kinetochore associated 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; NDC80 homolog, kinetochore complex component; NDC80 kinetochore complex component homolog; nuclear protein; Retinoblastoma-associated protein HEC; unnamed protein product
Gene Aliases: 2610020P18Rik; AV139730; HEC; HEC1; HsHec1; hsNDC80; KNTC2; NDC80; TID3
UniProt ID: (Human) O14777, (Mouse) Q9D0F1
Entrez Gene ID: (Human) 10403, (Rat) 301701, (Mouse) 67052
mitotic sister chromatid segregation
establishment of mitotic spindle orientation
mitotic cell cycle
nuclear division
spindle organization
mitotic spindle organization
spindle assembly involved in female meiosis I
chromosome segregation
mitotic spindle assembly checkpoint
meiotic G2/MI transition
attachment of spindle microtubules to kinetochore
skeletal muscle satellite cell proliferation
regulation of protein stability
chromosome organization
centrosome duplication
cell division
metaphase plate congression
attachment of mitotic spindle microtubules to kinetochore
kinetochore organization
positive regulation of mitotic cell cycle spindle assembly checkpoint
nuclear chromosome segregation
cell cycle
mitotic nuclear division
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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