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Invitrogen
Phospho-SMAD1 (Ser465) Polyclonal Antibody
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Product Details
PA5-36771
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 465
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 60 kDa.
Purity is >95% by SDS-PAGE.
Target Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; Sma and Mad homolog; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1; unnamed protein product
Gene Aliases: AI528653; BSP-1; BSP1; JV4-1; JV41; Mad1; MADH1; MADR1; Mlp1; MusMLP; SMAD1
UniProt ID: (Human) Q15797, (Mouse) P70340, (Rat) P97588
Entrez Gene ID: (Human) 4086, (Mouse) 17125, (Rat) 25671
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
DEAD/H-box RNA helicase binding
protein kinase binding
ubiquitin protein ligase binding
identical protein binding
sequence-specific DNA binding
metal ion binding
co-SMAD binding
I-SMAD binding
primary miRNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein homodimerization activity
protein heterodimerization activity
DNA-binding transcription factor
gene-specific transcriptional regulator
MAPK cascade
ossification
osteoblast differentiation
ureteric bud development
mesodermal cell fate commitment
osteoblast fate commitment
cardiac conduction system development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
cellular iron ion homeostasis
inflammatory response
signal transduction
transforming growth factor beta receptor signaling pathway
gamete generation
cell proliferation
negative regulation of cell proliferation
anatomical structure morphogenesis
embryonic pattern specification
positive regulation of gene expression
cell differentiation
BMP signaling pathway
midbrain development
hindbrain development
primary miRNA processing
developmental process
homeostatic process
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
negative regulation of muscle cell differentiation
cartilage development
cardiac muscle cell proliferation
bone development
SMAD protein signal transduction
positive regulation of cartilage development
cellular response to growth factor stimulus
transforming growth factor beta receptor superfamily signaling pathway
positive regulation of dendrite development
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of sprouting angiogenesis
anti-Mullerian hormone signaling pathway
protein phosphorylation
SMAD protein complex assembly
response to drug
positive regulation of cell differentiation
cellular response to organic cyclic compound
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
kidney development
response to organonitrogen compound
negative regulation of muscle cell apoptotic process
wound healing
positive regulation of dendrite morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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