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Invitrogen

Phospho-SMAD1 (Ser187) Polyclonal Antibody

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Phospho-SMAD1 (Ser187) Polyclonal Antibody

Product Details

PA5-36876

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Immunoprecipitation (IP)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 187
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553795

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 60 kDa.

Purity is >95% by SDS-PAGE.

Target Information

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; Sma and Mad homolog; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1; unnamed protein product

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Gene Aliases: AI528653; BSP-1; BSP1; JV4-1; JV41; Mad1; MADH1; MADR1; Mlp1; MusMLP; SMAD1

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UniProt ID: (Human) Q15797, (Mouse) P70340, (Rat) P97588

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Entrez Gene ID: (Human) 4086, (Mouse) 17125, (Rat) 25671

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding DEAD/H-box RNA helicase binding protein kinase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding metal ion binding co-SMAD binding I-SMAD binding primary miRNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein homodimerization activity protein heterodimerization activity DNA-binding transcription factor gene-specific transcriptional regulator
Process(es)
MAPK cascade ossification osteoblast differentiation ureteric bud development mesodermal cell fate commitment osteoblast fate commitment cardiac conduction system development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis inflammatory response signal transduction transforming growth factor beta receptor signaling pathway gamete generation cell proliferation negative regulation of cell proliferation anatomical structure morphogenesis embryonic pattern specification positive regulation of gene expression cell differentiation BMP signaling pathway midbrain development hindbrain development primary miRNA processing developmental process homeostatic process positive regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter stem cell differentiation negative regulation of muscle cell differentiation cartilage development cardiac muscle cell proliferation bone development SMAD protein signal transduction positive regulation of cartilage development cellular response to growth factor stimulus transforming growth factor beta receptor superfamily signaling pathway positive regulation of dendrite development positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of sprouting angiogenesis anti-Mullerian hormone signaling pathway protein phosphorylation SMAD protein complex assembly response to drug positive regulation of cell differentiation cellular response to organic cyclic compound cellular response to BMP stimulus positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus kidney development response to organonitrogen compound negative regulation of muscle cell apoptotic process wound healing positive regulation of dendrite morphogenesis
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