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Invitrogen
GCLC Polyclonal Antibody
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FIGURE: 1 / 5
GCLC Antibody (PA5-44190) in WB
Western blot was performed using Anti-GCLC Polyclonal Antibody (Product # PA5-44190) and a 72 kDa band corresponding to GCLC was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), Jurkat (Lane 4), Caco-2 (Lane 5), Mouse Liver (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting Sy... View More
Product Details
PA5-44190
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide directed towards the middle region of human GCLC
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity Chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Peptide sequence: RISKSRYDSI DSYLSKCGEK YNDIDLTIDK EIYEQLLQEG IDHLLAQHVA
Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
Target Information
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; unnamed protein product
Gene Aliases: CNSHA7; D9Wsu168e; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC
UniProt ID: (Human) P48506, (Mouse) P97494
Entrez Gene ID: (Human) 2729, (Mouse) 14629
cysteine metabolic process
glutamate metabolic process
glutathione metabolic process
glutathione biosynthetic process
response to oxidative stress
response to nutrient
response to heat
response to xenobiotic stimulus
response to hormone
response to activity
L-ascorbic acid metabolic process
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
cellular response to insulin stimulus
cellular response to hepatocyte growth factor stimulus
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
cellular response to fibroblast growth factor stimulus
response to human chorionic gonadotropin
cell redox homeostasis
negative regulation of transcription, DNA-templated
response to arsenic-containing substance
response to cadmium ion
response to nitrosative stress
regulation of mitochondrial depolarization
response to interleukin-1
cellular response to mechanical stimulus
cellular response to glucose stimulus
cellular response to follicle-stimulating hormone stimulus
cellular response to thyroxine stimulus
regulation of blood vessel diameter
negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
negative regulation of hepatic stellate cell activation
negative regulation of extrinsic apoptotic signaling pathway
apoptotic mitochondrial changes
regulation of blood vessel size
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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