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Invitrogen
LGMN Polyclonal Antibody
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FIGURE: 1 / 1
LGMN Antibody (PA5-47244) in WB
Western blot analysis of LGMN in J774A.1 mouse reticulum cell sarcoma macrophage cell line and Neuro‚2A mouse neuroblastoma cell line. Samples were incubated in LGMN polyclonal antibody (Product # PA5-47244) using a dilution of 0.2 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. Specific bands were detected for Legumain/Asparaginyl Endopeptidase at approximately 37 and 56 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47244
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Legumain Val18-Tyr435
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 50% cross-reactivity with recombinant human Legumain is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: asparaginyl endopeptidase; Legumain; preprolegumain; Protease, cysteine 1; protease, cysteine, 1; PRSC1EC 3.4.22.34
Gene Aliases: AEP; AI746452; AU022324; Lgmn; Prsc1
UniProt ID: (Mouse) O89017
Entrez Gene ID: (Mouse) 19141
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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