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Invitrogen
EMP Polyclonal Antibody
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FIGURE: 1 / 4
EMP Antibody (PA5-47914) in ICC/IF
Immunocytochemistry analysis of EMP in immersion fixed Jurkat human acute T cell leukemia cell line. Samples were incubated in EMP polyclonal antibody (Product # PA5-47914) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47914
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human EMP/MAEA Met12-Ser66
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes, this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 5 protein; E3 ubiquitin-protein transferase MAEA; Erythroblast macrophage protein; GID complex subunit 9, FYV10 homolog; HLC-10; Human lung cancer oncogene 10 protein; lung cancer-related protein 10; Macrophage erythroblast attacher; P44EMLP; PIG5; unnamed protein product
Gene Aliases: 1110030D19Rik; EMLP; EMP; GID9; HLC-10; HLC10; MAEA; P44EMLP; PIG5
UniProt ID: (Human) Q7L5Y9, (Rat) Q5RKJ1, (Mouse) Q4VC33
Entrez Gene ID: (Human) 10296, (Rat) 298982, (Mouse) 59003
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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