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Invitrogen
ID3 Polyclonal Antibody
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Product Details
PA5-50683
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues near the N terminal of human inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Amount
240 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total ID3 protein.
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb25; Class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID-like protein inhibitor HLH 1R21; ID-like protein inhibitor HLH 462; Inhibitor of differentiation 3; Inhibitor of DNA binding 3; Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein; inhibitor of DNA binding 3, HLH protein; unnamed protein product
Gene Aliases: 1R21; BHLHB25; HEIR-1; HEIR1; Hlh462; Id-3; ID3; Idb3
UniProt ID: (Human) Q02535, (Rat) P41138, (Mouse) P41133
Entrez Gene ID: (Human) 3399, (Rat) 25585, (Mouse) 15903
transcription corepressor activity
protein binding
protein domain specific binding
bHLH transcription factor binding
protein dimerization activity
transcription regulator inhibitor activity
leptomycin B binding
transcription factor activity, sequence-specific DNA binding
transcription factor binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
metanephros development
regulation of DNA replication
central nervous system development
heart development
muscle organ development
circadian rhythm
positive regulation of gene expression
negative regulation of gene expression
neuron differentiation
epithelial cell differentiation
notochord development
odontogenesis
positive regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of myoblast differentiation
negative regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
rhythmic process
cellular response to leptomycin B
transcription, DNA-templated
positive regulation of cell proliferation
response to wounding
negative regulation of cell differentiation
regulation of cell cycle
regulation of transcription, DNA-templated
multicellular organism development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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