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Invitrogen
SMARCA5 Polyclonal Antibody
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FIGURE: 1 / 14
SMARCA5 Antibody (PA5-52601) in ICC/IF
Immunofluorescence analysis of SMARCA5 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SMARCA5 Polyclonal Antibody (Product # PA5-52601) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790)... View More
Product Details
PA5-52601
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human SMARCA5. Recombinant protein control fragment (Product #RP-89367).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SSAAEPPPPP PPESAPSKPA ASIASGGSNS SNKGGPEGVA AQAVASAASA GPADAEMEEI FDDASPGKQK EIQEPDPTYE EKMQTDRANR FEYLLKQTEL FAHFIQPA
Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 46%.
Target Information
SMARCA5 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA5 is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hSNF2H; mSnf2h; SMARCA5; Sucrose nonfermenting protein 2 homolog; sucrose nonfermenting-like 5; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; unnamed protein product
Gene Aliases: 4933427E24Rik; D030040M08Rik; D330027N15Rik; hISWI; hSNF2H; ISWI; MommeD4; SMARCA5; SNF2H; WCRF135
UniProt ID: (Human) O60264, (Mouse) Q91ZW3
Entrez Gene ID: (Human) 8467, (Rat) 307766, (Mouse) 93762
DNA binding
chromatin binding
helicase activity
protein binding
ATP binding
hydrolase activity
ATPase activity
nucleosome binding
histone binding
ATP-dependent chromatin remodeler activity
nucleosome array spacer activity
histone octamer slider activity
molecular_function
nucleotide binding
nucleic acid binding
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
DNA helicase
chromatin silencing at rDNA
regulation of DNA replication
DNA repair
chromatin organization
nucleosome assembly
chromatin remodeling
methylation-dependent chromatin silencing
DNA-templated transcription, initiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
negative regulation of transcription from RNA polymerase I promoter
heterochromatin assembly
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase I promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
antiviral innate immune response
negative regulation of mitotic chromosome condensation
cellular response to leukemia inhibitory factor
double-strand break repair
chromatin assembly or disassembly
biological_process
metabolic process
nucleosome positioning
ATP-dependent chromatin remodeling
embryo development
chromatin modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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