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FIGURE: 1 / 5
Spastin Antibody (PA5-53581) in WB
Western blot of SPAST was performed by loading 80 µg of WT (lanes 1/3) and SPAST CRISPR KO (lanes 2/4) HEK293T or HAP1 cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. SPAST was detected at approximately 67 kDa (designated by the black arrow) using a SPAST polyclonal antibody (Product # PA5-53581) at a dilution of 1:500 in 5% BSA in TBS with 0.1% Tween 20 ... View More
Product Details
PA5-53581
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Spastin. Recombinant protein control fragment (Product #RP-89991).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: KRKDPLTHTS NSLPRSKTVM KTGSAGLSGH HRAPSYSGLS MVSGVKQGSG PAPTTHKGTP KTNRTNKPST PTTATRKKKD LKNFRNVDSN LANLIMNEIV DNGTAVKFDD IAGQDLAKQA LQEIVILPSL RPELFTGL
Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.
Target Information
This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: fidgetin-like 1; KIAA183; spastic paraplegia 4 (autosomal dominant; spastin); Spastic paraplegia 4 protein; Spastin; unnamed protein product
Gene Aliases: ADPSP; FSP2; KIAA1083; SPAST; SPG4
UniProt ID: (Human) Q9UBP0
Entrez Gene ID: (Human) 6683
mitotic cytokinesis
microtubule bundle formation
ER to Golgi vesicle-mediated transport
mitotic nuclear envelope reassembly
nervous system development
axonogenesis
anterograde axonal transport
exit from mitosis
axonal transport of mitochondrion
central nervous system neuron axonogenesis
cell differentiation
positive regulation of microtubule depolymerization
nuclear envelope reassembly
positive regulation of cytokinesis
cytokinetic process
protein hexamerization
microtubule severing
mitotic spindle disassembly
protein homooligomerization
cell division
cytoskeleton-dependent cytokinesis
membrane fission
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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