Search
Invitrogen
CH25H Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
CH25H Antibody (PA5-72349) in WB
Western blot analysis of CH25H in various lysates. Samples were incubated with CH25H polyclonal antibody (Product # PA5-72349) using a dilution of 1:1,000-1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Human brain lysate; Lane 2: Mouse heart lysate; Lane 3: Mouse lung lysate. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-72349
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 47-76 amino acids from human CH25H
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
400 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 1.14.99.38; Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase; h25OH; m25OH; unnamed protein product
Gene Aliases: AI462618; C25H; CH25H; m25OH
UniProt ID: (Human) O95992, (Mouse) Q9Z0F5
Entrez Gene ID: (Human) 9023, (Mouse) 12642
lipid metabolic process
steroid biosynthetic process
steroid metabolic process
cholesterol metabolic process
lipid biosynthetic process
sterol biosynthetic process
response to type I interferon
B cell chemotaxis
negative regulation of cholesterol metabolic process
negative regulation of fusion of virus membrane with host plasma membrane
fatty acid biosynthetic process
oxidation-reduction process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support