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Invitrogen
Hemoglobin gamma A Polyclonal Antibody
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FIGURE: 1 / 6
Hemoglobin gamma A Antibody (PA5-77998) in WB
Western blot was performed using Anti-Hemoglobin gamma A Polyclonal Antibody(Product # PA5-77998) and a 15kDa band corresponding to Hemoglobin gamma A was observed in K-562 and not in THP-1 and Jurkat which are reported to be negative. Membrane enriched extracts (30 µg lysate) of K-562 (Lane 1), THP-1 (Lane 2) and Jurkat (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Produc... View More
Product Details
PA5-77998
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human Hemoglobin gamma A Recombinant protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: K562
Predicted Reactivity: Chimpanzee (99%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon - gamma-G - gamma-A - delta - beta-3'.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-gamma globin; epsilon 3 globin; fetal hemoglobin F subunit gamma 1; gamma A hemoglobin; gamma globin; Gamma-1-globin; Hb F Agamma; Hemoglobin gamma-1 chain; Hemoglobin gamma-A chain; Hemoglobin subunit gamma-1; hemoglobin Y beta-like embryonic chain; hemoglobin Y, beta-like embryonic chain; hemoglobin, epsilon 1; hemoglobin, gamma A; hemoglobin, gamma, regulator of; unnamed protein product
Gene Aliases: Hbb-y; Hbe1; HBG-T2; HBG1; HBGA; HBGR; HSGGL1; PRO2979
UniProt ID: (Human) P69891
Entrez Gene ID: (Human) 3047, (Rat) 94164
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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