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Invitrogen

EHMT2 Polyclonal Antibody

Advanced Verification
1 Published Figure
2 References
View all (20) EHMT2 antibodies

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Product Details

PA5-78347

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-

ChIP assay (ChIP)

Assay-Dependent
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human G9a. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.34 mg/mL

Amount

134 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735793

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12

Predicted Reactivity: Rat (99%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ankyrin repeat-containing protein; DKFZp686H08213; Euchromatic histone-lysine N-methyltransferase 2; FLJ35547; G9A histone methyltransferase; H3 ly; H3-K9-Histone-Methyl-Transferase; H3-K9-HMTase 3; Histone H3-K9 methyltransferase 3; Histone-lysine N-methyltransferase EHMT2; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; HLA-B associated transcript 8, rat orthologue; HLA-B-associated transcript 8; Lysine N-methyltransferase 1C; NG36/G9a; Protein G9a; unnamed protein product

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Gene Aliases: BAT8; C6orf30; D17Ertd710e; EHMT2; G9A; GAT8; KMT1C; NG36

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UniProt ID: (Human) Q96KQ7, (Mouse) Q9Z148

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Entrez Gene ID: (Human) 10919, (Mouse) 110147, (Rat) 361798

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding transcription corepressor binding p53 binding protein binding methyltransferase activity zinc ion binding protein-lysine N-methyltransferase activity transferase activity histone methyltransferase activity metal ion binding histone methyltransferase activity (H3-K9 specific) histone methyltransferase activity (H3-K27 specific) C2H2 zinc finger domain binding histone H3K56 methyltransferase activity histone H3 methyltransferase activity histone H3K9me2 methyltransferase activity promoter-specific chromatin binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity histone-lysine N-methyltransferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter regulation of DNA replication DNA methylation regulation of transcription from RNA polymerase II promoter synaptonemal complex assembly germ cell development spermatid development long-term memory fertilization DNA methylation on cytosine within a CG sequence chromatin modification histone methylation peptidyl-lysine dimethylation methylation histone lysine methylation organ growth cellular response to drug regulation of DNA methylation histone H3-K9 methylation regulation of histone H3-K4 methylation regulation of histone H3-K9 methylation histone H3-K27 methylation peptidyl-lysine methylation response to nutrient levels response to ethanol response to fungicide chromatin organization methylation-dependent chromatin silencing cellular response to starvation phenotypic switching regulation of gene expression, epigenetic hypermethylation of CpG island behavioral response to cocaine oocyte development neuron fate specification cellular response to cocaine negative regulation of autophagosome assembly
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