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Invitrogen
Phospho-PDCD4 (Ser457) Polyclonal Antibody
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FIGURE: 1 / 6
Phospho-PDCD4 (Ser457) Antibody (PA5-78408) in IHC (P)
Phospho-PDCD4 (Ser457) Polyclonal Antibody detects PDCD4 (phospho Ser457) protein at nucleus in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Phospho-PDCD4 (Ser457) Polyclonal Antibody (Product # PA5-78408) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-78408
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser457 of human PDCD4. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
2.03 mg/mL
Amount
203 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: NIH-3T3(starvation 16 hours and 5 ng/mL PDGF treatment for 24 hr)
Predicted Reactivity: Human (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Death up-regulated gene protein; death-upregulated; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; RP11-348N5.4; Topoisomerase-inhibitor suppressed protein; unnamed protein product
Gene Aliases: D19Ucla1; Dug; H731; Ma3; PDCD4; Tis
UniProt ID: (Human) Q53EL6, (Rat) Q9JID1, (Mouse) Q61823
Entrez Gene ID: (Human) 27250, (Rat) 64031, (Mouse) 18569
apoptotic process
BMP signaling pathway
negative regulation of JUN kinase activity
negative regulation of transcription, DNA-templated
positive regulation of inflammatory response
epithelial to mesenchymal transition involved in cardiac fibroblast development
cellular response to lipopolysaccharide
negative regulation of cytokine production involved in inflammatory response
positive regulation of NIK/NF-kappaB signaling
negative regulation of vascular smooth muscle cell proliferation
negative regulation of myofibroblast differentiation
negative regulation of vascular smooth muscle cell differentiation
positive regulation of vascular associated smooth muscle cell apoptotic process
positive regulation of endothelial cell apoptotic process
cell aging
positive regulation of smooth muscle cell apoptotic process
negative regulation of apoptotic process
regulation of protein metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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