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          • Primary Antibodies ›
          • CLPP Antibodies

          Invitrogen

          CLPP Polyclonal Antibody

          1 Reference
          View all (29) CLPP antibodies

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          Cite CLPP Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (9)
          CLPP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CLPP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          CLPP Antibody (PA5-79051) in ICC/IF

          Immunocytochemistry analysis of CLPP using anti-CLPP antibody (Product # PA5-79051) . CLPP was detected in a section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CLPP antibody (Product # PA5-79051) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CLPP Antibody in Immunocytochemistry (ICC/IF)
          CLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CLPP Antibody in Western Blot (WB)
          CLPP Antibody in Flow Cytometry (Flow)
          CLPP Antibody in Flow Cytometry (Flow)
          CLPP Polyclonal Antibody

          Product Details

          PA5-79051

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          0.5-1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived human CLPP recombinant protein (Position: A184-H247).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2746167

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human PC-3 whole cell, human HEK293 whole cell, human MCF-7 whole cell, human THP-1 whole cell, rat pancreas tissue, rat lung tissue, mouse pancreas tissue. IHC: human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovarian cancer tissue, human gallbladder adenocarcinoma tissue. ICC/IF: MCF-7 cell. Flow: U937 cell.

          Target Information

          The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ATP-dependent Clp protease proteolytic subunit, mitochondrial; ATP-dependent protease ClpAP, proteolytic subunit, human; Caseinolytic mitochondrial matrix peptidase proteolytic subunit; caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; Endopeptidase Clp; putative ATP-dependent Clp protease proteolytic subunit, mitochondrial; unnamed protein product

          View more View less

          Gene Aliases: AU019820; CLPP; D17Wsu160e; DFNB81; PRLTS3

          View more View less

          UniProt ID: (Human) Q16740, (Mouse) O88696

          View more View less

          Entrez Gene ID: (Human) 8192, (Rat) 301117, (Mouse) 53895

          View more View less

          Function(s)
          endopeptidase activity ATP-dependent peptidase activity serine-type endopeptidase activity protein binding peptidase activity serine-type peptidase activity hydrolase activity identical protein binding ATPase binding serine protease
          Process(es)
          proteolysis misfolded or incompletely synthesized protein catabolic process membrane protein proteolysis mitochondrial protein catabolic process proteolysis involved in cellular protein catabolic process protein homooligomerization
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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