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Invitrogen
NOX2 Polyclonal Antibody
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FIGURE: 1 / 6
NOX2 Antibody (PA5-79118) in IHC (P)
Immunohistochemistry (Paraffin) analysis of NOX2 in paraffin-embedded section of human tonsil tissue using NOX2 Polyclonal Antibody (Product # PA5-79118). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4°C. ... View More
Product Details
PA5-79118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NOX2 (442-459aa YWLCRDTHAFEWFADLLQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Raji whole cell, human Jurkat whole cell, human MCF-7 whole cell, rat RH35 whole cell, mouse RAW264.7 whole cell. IHC: human tonsil tissue.
Target Information
Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet possess distinct expression patterns. gp91-phox expresses in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, with low expression in the uterus and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 beta polypeptide; Cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; endothelial type gp91-phox; GP91 PHOX; gp91-1; gp91-phox; GP911; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; p91PHOX; Superoxide-generating NADPH oxidase heavy chain subunit; unnamed protein product
Gene Aliases: AMCBX2; C88302; CGD; CGD91-phox; CGDX; CYBB; Cyd; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX
UniProt ID: (Human) P04839, (Mouse) Q61093
Entrez Gene ID: (Human) 1536, (Rat) 66021, (Mouse) 13058
protein binding
electron carrier activity
NAD(P)H oxidase activity
superoxide-generating NADPH oxidase activity
oxidoreductase activity
heme binding
voltage-gated proton channel activity
metal ion binding
protein heterodimerization activity
flavin adenine dinucleotide binding
oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
NADPH binding
FAD binding
superoxide-generating NADPH oxidase activity
voltage-gated ion channel activity
N-ethylmaleimide reductase activity
reduced coenzyme F420 dehydrogenase activity
sulfur oxygenase reductase activity
malolactic enzyme activity
NADPH:sulfur oxidoreductase activity
epoxyqueuosine reductase activity
oxidase
superoxide metabolic process
ion transport
defense response
inflammatory response
response to nutrient
response to xenobiotic stimulus
positive regulation of tumor necrosis factor production
ion transmembrane transport
superoxide anion generation
innate immune response
response to ethanol
respiratory burst
positive regulation of angiogenesis
hydrogen peroxide biosynthetic process
cellular response to cadmium ion
cellular response to ethanol
cellular response to hypoxia
hypoxia-inducible factor-1alpha signaling pathway
response to aldosterone
cellular response to L-glutamine
response to angiotensin
response to drug
oxidation-reduction process
transport
regulation of ion transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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