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Invitrogen
ISG15 Polyclonal Antibody
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FIGURE: 1 / 6
ISG15 Antibody (PA5-79522) in ICC/IF
Immunofluorescence analysis of ISG15 was performed using 70% confluent log phase A549 cells treated with IFN alpha (1000U/mL for 48h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ISG15 Rabbit Polyclonal Antibody (Product # PA5-79522) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Se... View More
Product Details
PA5-79522
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ISG15 recombinant protein (Position: G2-G157).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: 22RV1 whole cell, HELA whole cell. IHC: human pancreatic cancer tissue.
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 17-kDa protein; hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
Gene Aliases: G1P2; hUCRP; IFI15; IMD38; IP17; ISG15; UCRP
UniProt ID: (Human) P05161
Entrez Gene ID: (Human) 9636
immune system process
regulation of immune system process
integrin-mediated signaling pathway
response to virus
response to bacterium
protein ubiquitination
modification-dependent protein catabolic process
positive regulation of bone mineralization
negative regulation of protein ubiquitination
ISG15-protein conjugation
positive regulation of protein oligomerization
regulation of interferon-gamma production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
response to type I interferon
defense response to bacterium
negative regulation of viral genome replication
innate immune response
positive regulation of erythrocyte differentiation
positive regulation of multicellular organismal process
defense response to virus
negative regulation of type I interferon-mediated signaling pathway
protein localization to mitochondrion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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