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          • Primary Antibodies ›
          • Lyn Antibodies

          Invitrogen

          Lyn Polyclonal Antibody

          Advanced Verification
          View all (41) Lyn antibodies

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          Cite Lyn Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (7)
          • Advanced Verification (1)
          Lyn Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Lyn Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Lyn Antibody (PA5-85319) in ICC/IF

          Immunofluorescence analysis of Lyn was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Lyn Polyclonal Antibody (Product # PA5-85319) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilutio... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Lyn Antibody in Immunocytochemistry (ICC/IF)
          Lyn Antibody in Immunocytochemistry (ICC/IF)
          Lyn Antibody in Western Blot (WB)
          Lyn Antibody in Western Blot (WB)
          Lyn Antibody in Western Blot (WB)
          Lyn Antibody in Western Blot (WB)
          Lyn Antibody in Immunoprecipitation (IP)
          Lyn Antibody

          Product Details

          PA5-85319

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -

          Immunoprecipitation (IP)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol, 1% BSA

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2792461

          Product Specific Information

          Keep as concentrated solution.

          Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (83%), Chicken (86%), Sheep (96%), Bovine (95%).

          Positive Control: HeLa, THP-1, CD44-knockout HeLa.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: FLJ26625; Lck/Yes-related novel protein tyrosine kinase; lyn protein non-receptor kinase; lyn tyrosine kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; unnamed protein product; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

          View more View less

          Gene Aliases: JTK8; LYN; p53Lyn; p56Lyn; SAIDV

          View more View less

          UniProt ID: (Human) P07948, (Rat) Q07014

          View more View less

          Entrez Gene ID: (Human) 4067, (Rat) 81515

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding platelet-derived growth factor receptor binding integrin binding protein binding ATP binding kinase activity transferase activity SH3 domain binding enzyme binding receptor activator activity ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding macromolecular complex binding ephrin receptor binding phosphoprotein binding scaffold protein binding phosphorylation-dependent protein binding phosphatidylinositol 3-kinase activator activity protein complex binding
          Process(es)
          DNA damage checkpoint cell morphogenesis B cell homeostasis regulation of cytokine production regulation of protein phosphorylation negative regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway hematopoietic progenitor cell differentiation adaptive immune response immune system process Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation innate immune response-activating signal transduction negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway negative regulation of inflammatory response to antigenic stimulus regulation of B cell apoptotic process protein phosphorylation autophagy response to stress inflammatory response cellular response to DNA damage stimulus response to sterol depletion signal transduction transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation response to xenobiotic stimulus response to toxic substance response to hormone response to carbohydrate positive regulation of neuron projection development oligodendrocyte development fatty acid transport peptidyl-tyrosine phosphorylation hemopoiesis erythrocyte differentiation eosinophil differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development T cell costimulation lipopolysaccharide-mediated signaling pathway response to insulin regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway negative regulation of toll-like receptor 4 signaling pathway cellular response to heat intracellular signal transduction interleukin-5-mediated signaling pathway Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis C-X-C chemokine receptor CXCR4 signaling pathway B cell proliferation positive regulation of phosphorylation response to amino acid regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of MAPK cascade response to peptide hormone innate immune response regulation of erythrocyte differentiation protein autophosphorylation ephrin receptor signaling pathway response to axon injury regulation of inflammatory response negative regulation of immune response B cell receptor signaling pathway regulation of B cell receptor signaling pathway negative regulation of B cell receptor signaling pathway leukocyte migration regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid cellular response to lipid regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation neuroinflammatory response negative regulation of intracellular signal transduction positive regulation of amyloid precursor protein catabolic process positive regulation of protein localization to plasma membrane positive regulation of dendritic cell apoptotic process chemotaxis response to organic cyclic compound cell migration cellular response to extracellular stimulus peptidyl-tyrosine autophosphorylation response to drug positive regulation of tyrosine phosphorylation of STAT protein positive regulation of phosphatidylinositol 3-kinase activity cytokine secretion regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation positive regulation of B cell receptor signaling pathway positive regulation of cellular component movement positive regulation of stress-activated protein kinase signaling cascade cellular response to peptide hormone stimulus
          It has to be done as per old AB suggested Products section.

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