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Invitrogen
VAMP2 Polyclonal Antibody
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FIGURE: 1 / 6
VAMP2 Antibody (PA5-85475) in ICC/IF
Immunofluorescence analysis of Vesicle-associated membrane protein 2 was performed using 70 confluent log phase PC-12 differentiated cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VAMP2 Polyclonal Antibody (Product # PA5-85475) at 1.03 mg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibod... View More
Product Details
PA5-85475
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human VAMP2 Recombinant protein.
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Amount
103 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (90%), Xenopus laevis (96%), Rhesus Monkey (100%), Bovine (99%).
Positive Control: mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Synaptobrevin; synaptobrevin 2; Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); synaptobrevin II; Synaptobrevin-2; unnamed protein product; vamp; VAMP-2; vesicle-associated membrane 2; Vesicle-associated membrane protein (synaptobrevin 2); Vesicle-associated membrane protein 2
Gene Aliases: NEDHAHM; RATVAMPB; RATVAMPIR; SYB; Syb-2; SYB2; sybII; VAMP-2; VAMP2
UniProt ID: (Human) P63027, (Mouse) P63044, (Rat) P63045
Entrez Gene ID: (Human) 6844, (Mouse) 22318, (Rat) 24803
exocytosis
vesicle fusion
response to glucose
protein transport
synaptic vesicle exocytosis
vesicle-mediated transport
calcium ion regulated exocytosis
regulation of exocytosis
cellular response to insulin stimulus
SNARE complex assembly
Golgi to plasma membrane protein transport
eosinophil degranulation
neutrophil degranulation
natural killer cell degranulation
synaptic vesicle endocytosis
long-term synaptic potentiation
regulation of vesicle-mediated transport
membrane fusion
protein-containing complex assembly
mucus secretion
positive regulation of intracellular protein transport
regulation of delayed rectifier potassium channel activity
regulation of histamine secretion by mast cell
protein complex assembly
regulation of calcium ion-dependent exocytosis
neurotransmitter secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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