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Invitrogen
TET2 Polyclonal Antibody
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FIGURE: 1 / 8
TET2 Antibody (PA5-85488) in ICC/IF
TET2 Polyclonal Antibody detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 protein stained by TET2 Polyclonal Antibody (Product # PA5-85488) diluted at 1:1,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Product Details
PA5-85488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.35 mg/mL
Amount
135 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Positive Control: 293T, A431, HeLa, HepG2, DDDDK-human TET2-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TET2 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydromethylcytosine (5hmC) and plays a key role in active DNA demethylation. TET2 has a preference for 5-hydroxymethylcytosine in CpG motifs. It mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC) and conversion of 5fC to 5-carboyxlcytosine (5caC). Conversion of 5mC into 5hmC, 5fC, and 5caC probably constitutes the first step in cytonsine demethylation. In addition to TET2's role in DNA demethylation, it is also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Mutations in the genes can result in Polycythemia vera and Myelodysplastic syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ20032; Methylcytosine dioxygenase TET2; MGC125715; OTTHUMP00000161869; probable methylcytosine dioxygenase TET2; Protein Ayu17 449; Protein Ayu17-449; ten-eleven translocation 2; Tet 2; tet oncogene 2; tet oncogene family member 2; unnamed protein product
Gene Aliases: Ayu17-449; E130014J05Rik; IMD75; KIAA1546; MDS; mKIAA1546; Nbla00191; TET2
UniProt ID: (Human) Q6N021, (Mouse) Q4JK59
Entrez Gene ID: (Human) 54790, (Mouse) 214133
DNA binding
protein binding
ferrous iron binding
zinc ion binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
methylcytosine dioxygenase activity
kidney development
myeloid progenitor cell differentiation
5-methylcytosine catabolic process
protein O-linked glycosylation
cell cycle
post-embryonic development
chromatin modification
5-methylcytosine metabolic process
cytosine metabolic process
hemoglobin metabolic process
hemopoiesis
myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
spleen development
homeostasis of number of cells
oxidation-reduction process
hematopoietic stem cell homeostasis
liver morphogenesis
DNA demethylation
histone H3-K4 trimethylation
leukocyte differentiation
chromatin organization
regulation of gene expression
positive regulation of macromolecule biosynthetic process
regulation of gene expression, epigenetic
hypomethylation of CpG island
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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