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Invitrogen
GEN1 Polyclonal Antibody
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FIGURE: 1 / 1
GEN1 Antibody (PA5-87557) in WB
Western blot analysis of GEN1 in extracts of various cell lines. Samples were incubated with GEN1 Polyclonal antibody (Product # PA5-87557) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-87557
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 669-908 of human GEN1 (NP_8724313)
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LQDLPLKERI FTKLSYPQDN LQPDVNLKTL SILSVKESCI ANSGSDCTSH LSKDLPGIPL QNESRDSKIL KGDQLLQEDY KVNTSVPYSV SNTVVKTCNV RPPNTALDHS RKVDMQTTRK ILMKKSVCLD RHSSDEQSAP VFGKAKYTTQ RMKHSSQKHN SSHFKESGHN KLSSPKIHIK ETEQCVRSYE TAENEESCFP DSTKSSLSSL QCHKKENNSG TCLDSPLPLR QRLKLRFQST; Positive Samples: H460, MCF-7, Mouse thymus, Mouse spleen; Cellular Location: Nucleus
Target Information
Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Flap endonuclease GEN homolog 1; Gen endonuclease homolog 1; Holliday junction resolvase; unnamed protein product
Gene Aliases: 5830483C08Rik; Gen; GEN1
UniProt ID: (Human) Q17RS7, (Mouse) Q8BMI4
Entrez Gene ID: (Human) 348654, (Mouse) 209334
magnesium ion binding
four-way junction DNA binding
DNA binding
catalytic activity
nuclease activity
endonuclease activity
crossover junction endodeoxyribonuclease activity
hydrolase activity
5'-flap endonuclease activity
protein homodimerization activity
metal ion binding
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
exodeoxyribonuclease
double-strand break repair via homologous recombination
DNA repair
DNA recombination
cellular response to DNA damage stimulus
regulation of centrosome duplication
replication fork processing
resolution of recombination intermediates
resolution of mitotic recombination intermediates
positive regulation of mitotic cell cycle spindle assembly checkpoint
nucleic acid phosphodiester bond hydrolysis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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