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Invitrogen

MIF Polyclonal Antibody

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MIF Polyclonal Antibody

Product Details

PA5-89525

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human MIF (NP_0024061)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.07 mg/mL

Amount

107 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2805629

Product Specific Information

Immunogen sequence: MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA; Positive Samples: HL-60, THP-1, SW620, Mouse kidney, Mouse lung, Mouse heart; Cellular Location: Cytoplasm

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Delayed early response protein 6; DER6; epididymis secretory sperm binding protein; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MGC107654; MIF; migration inhibitory factor; Phenylpyruvate tautomerase; unnamed protein product

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Gene Aliases: GIF; GLIF; MIF; MMIF

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UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904

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Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683

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Function(s)
protease binding dopachrome isomerase activity cytokine activity cytokine receptor binding protein binding isomerase activity chemoattractant activity identical protein binding phenylpyruvate tautomerase activity receptor binding protein complex binding decarboxylase
Process(es)
prostaglandin biosynthetic process positive regulation of cytokine production immune system process negative regulation of mature B cell apoptotic process inflammatory response cell surface receptor signaling pathway positive regulation of cell proliferation negative regulation of gene expression negative regulation of macrophage chemotaxis carboxylic acid metabolic process DNA damage response, signal transduction by p53 class mediator negative regulation of cell migration positive regulation of B cell proliferation positive regulation of lipopolysaccharide-mediated signaling pathway positive regulation of tumor necrosis factor production negative regulation of myeloid cell apoptotic process regulation of cell proliferation positive regulation of phosphorylation regulation of macrophage activation negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator innate immune response positive regulation of fibroblast proliferation positive chemotaxis negative regulation of protein metabolic process positive regulation of prostaglandin secretion involved in immune response positive regulation of myeloid leukocyte cytokine production involved in immune response protein homotrimerization positive regulation of ERK1 and ERK2 cascade regulation of cellular response to stress positive regulation of arachidonic acid secretion cellular senescence positive regulation of cAMP/PKA signal transduction negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of cellular senescence positive regulation of protein phosphorylation brain renin-angiotensin system positive regulation of acute inflammatory response positive regulation of adaptive immune response spermatogenesis cell aging cell proliferation positive regulation of protein kinase A signaling positive regulation of smooth muscle cell migration negative regulation of cellular protein metabolic process positive regulation of peptidyl-serine phosphorylation drinking behavior positive regulation of potassium ion transport positive regulation of MAP kinase activity positive regulation of glycolytic process positive regulation of glucose import positive regulation of axon regeneration positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation positive regulation of immune response negative regulation of cell cycle arrest negative regulation of cell aging hair follicle development brain development aging metabolic process response to heat response to mechanical stimulus response to hormone response to inorganic substance response to estradiol response to lipopolysaccharide response to progesterone response to menaquinone response to insulin response to vitamin E wound healing response to drug skin development response to glucocorticoid cellular response to hydrogen peroxide cellular response to hypoxia
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