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Invitrogen

PKC beta-1 Polyclonal Antibody

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PKC beta-1 Polyclonal Antibody

Product Details

PA5-95606

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence of human PKC beta 1/PRKCB (NFDKEFTRQPVELTPTDKLFIMNLDQNE).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807408

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell lysates, human HEL whole cell lysates, human Jurkat whole cell lysates, rat brain tissue lysates, rat PC-12 whole cell lysates, mouse brain tissue lysates. IHC: mouse brain tissue. Flow: Jurkat cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

The PKC family of serine/threonine kinases, including PRKCB1 (PKC beta I), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PKC-B; PKC-beta; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; unnamed protein product

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Gene Aliases: A130082F03Rik; PKC-Beta; PKCB; PKCbeta; PKCI(2); PRKCB; PRKCB1; PRKCB2

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UniProt ID: (Human) P05771, (Mouse) F2Z441, (Rat) P68403

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Entrez Gene ID: (Human) 5579, (Mouse) 18751, (Rat) 25023

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Function(s)
nucleotide binding chromatin binding transcription coactivator activity protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein kinase C binding calcium channel regulator activity protein binding ATP binding zinc ion binding kinase activity transferase activity histone kinase activity (H3-T6 specific) histone binding metal ion binding androgen receptor binding protein serine kinase activity transferase activity, transferring phosphorus-containing groups ligand-dependent nuclear receptor transcription coactivator activity non-receptor serine/threonine protein kinase protein modifying enzyme
Process(es)
adaptive immune response immune system process chromatin organization chromatin remodeling regulation of transcription from RNA polymerase II promoter protein phosphorylation calcium ion transport cellular calcium ion homeostasis apoptotic process mitotic nuclear envelope disassembly signal transduction phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway regulation of glucose transport negative regulation of glucose transport positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of insulin secretion intracellular signal transduction B cell activation lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis positive regulation of transcription, DNA-templated negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway regulation of transport protein kinase C signaling cellular response to carbohydrate stimulus presynaptic modulation of chemical synaptic transmission regulation of synaptic vesicle exocytosis response to hypoxia transcription, DNA-templated regulation of transcription, DNA-templated regulation of dopamine secretion phosphorylation chromatin modification histone H3-T6 phosphorylation regulation of growth response to drug positive regulation of B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity response to glucose dibenzo-p-dioxin metabolic process response to vitamin D positive regulation of odontogenesis of dentin-containing tooth response to ethanol
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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