Search
Invitrogen
CDKN2B Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
CDKN2B Antibody (PA5-96390) in WB
Western blot analysis of CDKN2B in extracts of various cell lines. Samples were incubated with CDKN2B Polyclonal antibody (Product # PA5-96390) using a dilution of 1:800, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96390
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-138 of human CDKN2B (NP_0049272)
Conjugate
Form
Liquid
Concentration
0.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, SW620, A-549; Cellular Location: Cytoplasm
Immunogen sequence: MREENKGMPS GGGSDEGLAS AAARGLVEKV RQLLEAGADP NGVNRFGRRA IQVMMMGSAR VAELLLLHGA EPNCADPATL TRPVHDAARE GFLDTLVVLH RAGARLDVRD AWGRLPVDLA EERGHRDVAG YLRTATGD
Target Information
The four known 15-to 19 kDa INK4 proteins (p15INK4b, p16INK4a, p18INK4c andp19INK4d) bind and inhibit CDK4 and CDK6 but not other CDKs, including cyclin E-Cdk2, cyclin A-Cdk2 and cyclin B-Cdk1. Like the three D-type cyclins, the INK4 genes are expressed in distinct tissue-specific patterns, suggesting that they are not strictly redundant. 5 p18INK4c is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CDK inhibitory protein; CDK4B inhibitor; CDKN2B; Cyclin-dependent kinase 4 inhibitor B; cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); cyclin-dependent kinase inhibitor p15; cyclin-dependent kinase inhibitor p15INK4b; cyclin-dependent kinase inhibitor protein; cyclin-dependent kinases 4 and 6 binding protein; MTS-2; Multiple tumor suppressor 2; p10; p14-INK4b; p14_CDK inhibitor; p14_INK4B; p15; p15 CDK inhibitor; p15-INK4b; p15_INK4B; p15INK4B
Gene Aliases: AV083695; CDK4I; CDKN2B; INK4B; MTS2; P15; p15(INK4b); p15INK4b; TP15
UniProt ID: (Human) P42772, (Mouse) P55271
Entrez Gene ID: (Human) 1030, (Mouse) 12579
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
megakaryocyte differentiation
positive regulation of transforming growth factor beta receptor signaling pathway
cellular response to nutrient
spleen development
negative regulation of epithelial cell proliferation
regulation of G0 to G1 transition
cellular response to cell-matrix adhesion
cellular senescence
regulation of G1/S transition of mitotic cell cycle
negative regulation of G1/S transition of mitotic cell cycle
regulation of cyclin-dependent protein serine/threonine kinase activity
G2/M transition of mitotic cell cycle
cell cycle
cell cycle arrest
mitotic cell cycle checkpoint
cellular response to extracellular stimulus
negative regulation of phosphorylation
negative regulation of protein serine/threonine kinase activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support