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SIRP alpha Polyclonal Antibody

View all (78) SIRP alpha (CD172a) antibodies

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SIRP alpha Polyclonal Antibody

Product Details

PA5-99184

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:400
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SIRPA(Accession P78324), corresponding to amino acid residues P460-K504.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818117

Product Specific Information

Antibody detects endogenous levels of total SHPS1.

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bit; Brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like protein with tyrosine - based activation motifs; brain immunological-like with tyrosine-based motifs; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD172 alpha; CD172 antigen-like family member A; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; Macrophage membrane protein MFP150; mSIRP-alpha1; MyD-1 antigen; p84; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); protein tyrosine phosphatase, non-receptor type substrate 1; Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); SHP substrate 1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; SIRP-alpha1; SIRPalpha; SIRPalpha2; tyrosine phosphatase SHP substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1; unnamed protein product

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Gene Aliases: AI835480; BIT; CD172A; MFR; MYD-1; MYD1; P84; PTPNS1; SHP-1; SHPS-1; SHPS1; SIRP; SIRPA

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UniProt ID: (Human) P78324, (Rat) P97710, (Mouse) P97797

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Entrez Gene ID: (Human) 140885, (Rat) 25528, (Mouse) 19261

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Function(s)
protein phosphatase inhibitor activity protein binding SH3 domain binding protein phosphatase binding GTPase regulator activity protein binding involved in heterotypic cell-cell adhesion protein binding involved in cell-cell adhesion protein antigen binding protein tyrosine kinase binding protein phosphorylated amino acid binding transmembrane signal receptor
Process(es)
cell adhesion regulation of gene expression cell migration negative regulation of lipopolysaccharide-mediated signaling pathway regulation of interferon-gamma production regulation of interleukin-1 beta production regulation of interleukin-6 production regulation of tumor necrosis factor production negative regulation of interferon-beta production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production heterotypic cell-cell adhesion monocyte extravasation negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of nitric oxide biosynthetic process regulation of nitric oxide biosynthetic process negative regulation of JNK cascade negative regulation of inflammatory response negative regulation of phagocytosis positive regulation of phagocytosis positive regulation of T cell activation cellular response to hydrogen peroxide negative regulation of ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to interleukin-12 negative regulation of macrophage inflammatory protein 1 alpha production negative regulation of chemokine (C-C motif) ligand 5 production cell-cell adhesion negative regulation of cytokine production involved in inflammatory response positive regulation of reactive oxygen species metabolic process hematopoietic progenitor cell differentiation phagocytosis, recognition phagocytosis, engulfment cytoskeleton organization actin filament organization cell-matrix adhesion movement of cell or subcellular component
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