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          • Primary Antibodies ›
          • ABL1/ABL2 Antibodies

          Invitrogen

          Phospho-ABL1/ABL2 (Tyr412) Polyclonal Antibody

          View all (6) ABL1/ABL2 antibodies

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          Cite Phospho-ABL1/ABL2 (Tyr412) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          Phospho-ABL1/ABL2 (Tyr412) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-ABL1/ABL2 (Tyr412) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Phospho-ABL1/ABL2 (Tyr412) Antibody (PA5-99344) in ICC/IF

          Immunofluorescent analysis of Phospho-c-Abl (Tyr412) in K562 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-c-Abl (Tyr412) polyclonal antibody (Product # PA5-99344) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-ABL1/ABL2 (Tyr412) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-ABL1/ABL2 (Tyr412) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-ABL1/ABL2 (Tyr412) Antibody in Western Blot (WB)
          Phospho-ABL1/ABL2 (Tyr412) Polyclonal Antibody

          Product Details

          PA5-99344

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human ABL1(Accession P00519), corresponding to amino acid residues around phosphorylated Tyr412.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          sequential chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2818277

          Product Specific Information

          Antibody detects endogenous levels of ABL1/2 only when phosphorylated at Tyr393, which site historically referenced as Tyr412.

          Target Information

          The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. In these leukemias the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein-tyrosine kinase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Abelson murine leukemia oncogene; Abelson murine leukemia viral oncogene homolog 1; Abelson murine leukemia viral oncogene homolog 2; Abelson tyrosine-protein kinase 1; Abelson tyrosine-protein kinase 2; Abelson-related gene protein; ABL kinase domain; abl protein; ABL protooncogene 1 nonreceptor tyrosine kinase; ABL1; abl2; arg tyrosine kinase; BCR-ABL1 p190; BCR/ABL e8a2 fusion; BCR/ABL1 e1a2 fusion protein; BCR/ABL1 fusion; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; c-abl oncogene 2, non-receptor tyrosine kinase; chimeric BCR::ABL1 protein; non-receptor tyrosine kinase; p150; p190; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; tyrosine kinase ARG; tyrosine kinase protein; Tyrosine-protein kinase ABL1; Tyrosine-protein kinase ABL2; Tyrosine-protein kinase ARG; unnamed protein product; v-abl Abelson murine leukemia oncogene 1; v-abl Abelson murine leukemia viral oncogene homolog 1; v-abl Abelson murine leukemia viral oncogene homolog 2; v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)

          View more View less

          Gene Aliases: AA536808; ABL; ABL1; ABL2; ABLL; AI325092; ARG; BCR-ABL; bcr/abl; c-ABL; c-ABL1; CHDSKM; E430008G22Rik; JTK7; p150; v-abl

          View more View less

          UniProt ID: (Human) P00519, (Human) P42684, (Mouse) P00520

          View more View less

          Entrez Gene ID: (Human) 25, (Human) 27, (Mouse) 11350, (Mouse) 11352

          View more View less

          Function(s)
          nucleotide binding magnesium ion binding four-way junction DNA binding bubble DNA binding phosphotyrosine binding DNA binding transcription coactivator activity actin monomer binding nicotinate-nucleotide adenylyltransferase activity protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein kinase C binding protein binding ATP binding enzyme activator activity kinase activity transferase activity enzyme binding protein kinase binding protein domain specific binding syntaxin binding manganese ion binding neuropilin binding SH2 domain binding protein serine/threonine kinase activator activity metal ion binding ephrin receptor binding actin filament binding mitogen-activated protein kinase binding proline-rich region binding delta-catenin binding supercoiled DNA binding sequence-specific double-stranded DNA binding receptor binding protein C-terminus binding transferase activity, transferring phosphorus-containing groups SH3 domain binding
          Process(es)
          cell adhesion signal transduction epidermal growth factor receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration regulation of autophagy positive regulation of neuron projection development peptidyl-tyrosine phosphorylation regulation of endocytosis regulation of cell adhesion regulation of actin cytoskeleton organization cellular response to oxidative stress negative regulation of Rho protein signal transduction exploration behavior protein modification process cellular response to retinoic acid positive regulation of establishment of T cell polarity regulation of cell motility positive regulation of T cell migration mitotic cell cycle neural tube closure B-1 B cell homeostasis immune effector process B cell proliferation involved in immune response transitional one stage B cell differentiation immune response-activating cell surface receptor signaling pathway DNA repair mismatch repair regulation of transcription, DNA-templated endocytosis phagocytosis autophagy apoptotic process cellular response to DNA damage stimulus response to oxidative stress integrin-mediated signaling pathway I-kappaB kinase/NF-kappaB signaling learning or memory associative learning intrinsic apoptotic signaling pathway in response to DNA damage response to xenobiotic stimulus post-embryonic development regulation of gene expression positive regulation of macromolecule biosynthetic process positive regulation of endothelial cell migration cerebellum morphogenesis negative regulation of cell-cell adhesion microspike assembly actin cytoskeleton organization actin filament polymerization neuron differentiation BMP signaling pathway negative regulation of BMP signaling pathway regulation of axon extension phospholipase C-inhibiting G-protein coupled receptor signaling pathway regulation of microtubule polymerization regulation of Cdc42 protein signal transduction positive regulation of interferon-gamma production positive regulation of interleukin-2 production cellular response to stress positive regulation of osteoblast proliferation substrate adhesion-dependent cell spreading response to endoplasmic reticulum stress intracellular signal transduction platelet-derived growth factor receptor-beta signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis neuropilin signaling pathway B cell proliferation regulation of cell proliferation signal transduction in response to DNA damage positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process endothelial cell migration regulation of T cell differentiation regulation of cell differentiation positive regulation of cell adhesion positive regulation of vasoconstriction negative regulation of mitotic cell cycle positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter alpha-beta T cell differentiation platelet-derived growth factor receptor signaling pathway ephrin receptor signaling pathway positive regulation of fibroblast proliferation spleen development thymus development activated T cell proliferation T cell receptor signaling pathway B cell receptor signaling pathway neuromuscular process controlling balance positive regulation of release of sequestered calcium ion into cytosol neuron apoptotic process negative regulation of ubiquitin-protein transferase activity myoblast proliferation positive regulation of stress fiber assembly establishment of localization in cell regulation of cell cycle mitochondrial depolarization positive regulation of focal adhesion assembly Bergmann glial cell differentiation cardiac muscle cell proliferation neuroepithelial cell differentiation cellular response to hydrogen peroxide ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade DNA conformation change cellular response to lipopolysaccharide cellular response to transforming growth factor beta stimulus response to epinephrine circulatory system development positive regulation of cell migration involved in sprouting angiogenesis cellular senescence vascular endothelial cell response to oscillatory fluid shear stress cell-cell adhesion regulation of postsynaptic specialization assembly positive regulation of phospholipase C/protein kinase C signal transduction positive regulation of dendrite development positive regulation of substrate adhesion-dependent cell spreading negative regulation of long-term synaptic potentiation cellular response to oxygen-containing compound regulation of hematopoietic stem cell differentiation regulation of intracellular signal transduction regulation of extracellular matrix organization positive regulation of extracellular matrix organization glomerular visceral epithelial cell apoptotic process cellular response to dopamine DN4 thymocyte differentiation protein localization to cytoplasmic microtubule plus-end regulation of modification of synaptic structure positive regulation of blood vessel branching negative regulation of double-strand break repair via homologous recombination positive regulation of Wnt signaling pathway, planar cell polarity pathway negative regulation of endothelial cell apoptotic process regulation of cellular senescence negative regulation of cellular senescence positive regulation of protein phosphorylation protein phosphorylation DNA damage induced protein phosphorylation phosphorylation cell migration peptidyl-tyrosine autophosphorylation negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response collateral sprouting positive regulation of peptidyl-tyrosine phosphorylation positive regulation of oxidoreductase activity negative regulation of protein serine/threonine kinase activity actin filament branching positive regulation of interleukin-2 secretion negative regulation of phospholipase C activity positive regulation of neuron death positive regulation of interferon-gamma secretion regulation of response to DNA damage stimulus substrate-dependent cell migration, cell extension actin filament organization learning visual learning positive regulation of phospholipase C activity neuron remodeling positive regulation of protein binding cellular protein localization dendrite morphogenesis actin filament bundle assembly dendritic spine maintenance
          It has to be done as per old AB suggested Products section.

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