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Invitrogen
TK1 Polyclonal Antibody
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FIGURE: 1 / 2
TK1 Antibody (PA5-99500) in ICC/IF
Immunofluorescent analysis of TK1 in MCF-7 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with TK1 polyclonal antibody (Product # PA5-99500) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-99500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TK1(Accession P04183), corresponding to amino acid residues M1-M37.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Thymidine Kinase 1/TK1.
Target Information
Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosolic thymidine kinase; EC 2.7.1.21; epididymis secretory sperm binding protein; KITH; thymidine kinase (EC 2.7.1.21); Thymidine kinase, cytosolic; unnamed protein product
Gene Aliases: D530002A18Rik; Tk; Tk-1; TK1; Tk1a; Tk1b; TK2
UniProt ID: (Human) P04183, (Rat) P27158, (Mouse) P04184
Entrez Gene ID: (Human) 7083, (Rat) 24834, (Mouse) 21877
nucleobase-containing compound metabolic process
deoxyribonucleoside monophosphate biosynthetic process
thymidine metabolic process
thymidine biosynthetic process
protein homotetramerization
DNA biosynthetic process
DNA synthesis involved in mitotic DNA replication
liver development
nucleotide biosynthetic process
response to toxic substance
skeletal muscle cell proliferation
phosphorylation
response to nutrient levels
response to copper ion
digestive tract development
response to cortisol
fetal process involved in parturition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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