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Invitrogen
Aequorea victoria GFP His-tag Recombinant Protein
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Product Details
RP-87944
Applications
Tested Dilution
Publications
Product Specifications
Species
Cnidarian
Expression System
E. coli
Amino acid sequence
Aequorea victoria GFP, amino acids Ser2-Lys238 (Accession # AAB65663) with an N-terminal His-tag
Tag
His-tag
Molecular weight
28.76 kDa
Class
Recombinant
Type
Protein
Purity
≥90% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC
Conjugate
Form
Lyophilized
Concentration
0.25 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
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