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Invitrogen
Mouse anti-Rat IgG2a Secondary Antibody, FITC
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FIGURE: 1 / 1
Rat IgG2a Secondary Antibody (SA1-25265) in Flow
Flow cytometry analysis of CCR7 on primary mouse splenocytes. Cells were blocked with an anti-CD16/32 monoclonal antibody (Fc receptor blocker) for 30 minutes on ice, and then stained with a CCR7 monoclonal antibody (Product # MA1-163), using 5 µg of antibody per 1x106 cells, in 1X PBS containing 5% FCS. After incubation of the primary antibody for 1 hour at 37C, the cells were stained with a FITC-conjugate... View More
Product Details
SA1-25265
Applications
Tested Dilution
Flow Cytometry (Flow)
5 µg/mL
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Secondary Antibody
Clone
MARG2a-1
Immunogen
Rat IgG2a
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
125 µg
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG2a
Antibody Form
Whole Antibody
Product Specific Information
SA1-25265 detects rat IgG2a, no cross reactivity is observed with other rat immunoglobulin subclasses.
Use 10 µL of the suggested dilution to label 10^6 cells in 100 µL.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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