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Invitrogen
Donkey anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, HRP
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FIGURE: 1 / 3
Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody (SA1-300) in WB
Chemiluminescent western blot was performed using Donkey anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # SA1-300). Membrane enriched extracts of MCF10A (Lane 1, 2, 3), SHSY5Y(Lane 4, 5, 6), HaCaT (Lane 7), Hela (Lane 8) and HEK-293 (Lane 9) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto anitrocellulose memb... View More
Product Details
SA1-300
Applications
Tested Dilution
Western Blot (WB)
1:250-1:5,000
Product Specifications
Species Reactivity
Chicken
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
32 µg/mL
Amount
8 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol, proprietary stabilizer
Contains
0.07% Kathon
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgY
Cross Adsorption
Against bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Product SA1-300 is conjugated to HRP and optimized for ECL applications. Product SA1-300 reacts with the heavy and light chains of the immunoglobulin molecule. Pre-absorbed with the following species proteins: Bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins.
Target Information
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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