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Invitrogen
Sheep anti-Goat IgG (H+L) Secondary Antibody
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FIGURE: 1 / 1
Goat IgG (H+L) Secondary Antibody (SA5-10321) in ELISA
ELISA analysis was performed using Goat IgG h+l antigen (500 ng/well, 60 min). Samples were blocked for 60 min with 1% BSA, incubated with AP Sheep anti-Goat IgG h+l HRP conjugated Sheep anti-Goat IgG (60 min), and followed by HRP conjugated Sheep anti-Goat IgG (Product # SA5-10321) for 60 min. Development was done with TMB substrate (10 min) and the reaction was stopped with 0.3M H2SO4. Results were read o... View More
Product Details
SA5-10321
Applications
Tested Dilution
Western Blot (WB)
Assay-dependent
Immunohistochemistry (IHC)
Assay-dependent
ELISA (ELISA)
Assay-dependent
Immunoelectrophoresis (IE)
Assay-dependent
Product Specifications
Species Reactivity
Goat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Goat IgG h+l
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
1 mg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
This antibody will react with goat IgG and with light chains common with other goat immunoglobulins as determined by ELISA and IEP techniques. This antibody may cross-react with IgG from other species.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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