Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
IL-12 p70 Recombinant Protein
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
IL-12 p70 Protein (SIL12) in ELISA Std
Sandwich ELISA analysis of human IL-12 was performed using a Human IL-12 (p70) Colorimetric ELISA kit (Product # EHIL12), by loading 50 ul per well of a biotinylated anti-human IL-12 antibody (Product # M121B) in duplicate at 0.03125 ug/ml, and 50 ul per well of Human IL-12 (p70) Recombinant Protein (Product # SIL12) in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/ml across a 7 ug/ml anti-human IL-12 (Product # M122) pre-coated plate and incubating for 3 hours at room temperature. The plate was washed then incubated with 100 ul per well of ... View More
Product Details
SIL12
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Concentration
1.5 ng/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
The Human IL-12 Standard is calibrated against NIBSC human IL-12 Reference Standard lot #95/544. 1 Thermo pg = 1.66 NIBSC pg = 0.0166 NIBSC unit.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
1500 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
Target Information
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support