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Gibco
Human Siglec-2 (CD22) (soluble) Recombinant Protein, PeproTech®
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Product Details
100-01-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
CHO cells
Amino acid sequence
SKWVFEHPET LYAWEGACVW IPCTYRALDG DLESFILFHN PEYNKNTSKF DGTRLYESTK DGKVPSEQKR VQFLGDKNKN CTLSIHPVHL NDSGQLGLRM ESKTEKWMER IHLNVSERPF PPHIQLPPEI QESQEVTLTC LLNFSCYGYP IQLQWLLEGV PMRQAAVTST SLTIKSVFTR SELKFSPQWS HHGKIVTCQL QDADGKFLSN DTVQLNVKHT PKLEIKVTPS DAIVREGDSV TMTCEVSSSN PEYTTVSWLK DGTSLKKQNT FTLNLREVTK DQSGKYCCQV SNDVGPGRSE EVFLQVQYAP EPSTVQILHS PAVEGSQVEF LCMSLANPLP TNYTWYHNGK EMQGRTEEKV HIPKILPWHA GTYSCVAENI LGTGQRGPGA ELDVQYPPKK VTTVIQNPMP IREGDTVTLS CNYNSSNPSV TRYEWKPHGA WEEPSLGVLK IQNVGWDNTT IACARCNSWC SWASPVALNV QYAPRDVRVR KIKPLSEIHS GNSVSLQCDF SSSHPKEVQF FWEKNGRLLG KESQLNFDSI SPEDAGSYSC WVNNSIGQTA SKAWTLEVLY APRRLRVSMS PGDQVMEGKS ATLTCESDAN PPVSHYTWFD WNNQSLPHHS QKLRLEPVKV QHSGAYWCQG TNSVGKGRSP LSTLTVYYSP ETIGRR
Molecular weight
75 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by its ability to inhibit the proliferation of Raji cells. The expected ED50 for this effect is 10-17 ug/ml.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
Recombinant Human CD22 is a soluble 75.0 kDa protein which corresponds to the extracellular domain of CD22.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
CD22, also known as BL-CAM, is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22alpha and CD22beta, with molecular weights of 130 and 140 kDa, respectively. These chains are produced by alternative splicing of the CD22 gene. CD22 is prominently expressed on mature B cells and B cell lymphomas, including hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin's lymphoma, but is negative in classical Hodgkin's lymphoma. The extracellular portion of CD22 contains seven Ig-like domains that preferentially bind alpha2,6-linked sialic acid moieties found on epithelial, endothelial, B, and T cells. This binding can be masked by cis interactions with sialic acids on the same cell surface. CD22 expression is limited to late stages of B-cell differentiation, making it useful for phenotyping mature leukemias. Intracellularly, CD22 features six tyrosine residues within immunotyrosine-based inhibitory motifs (ITIM) and activation-like motifs. These residues are phosphorylated upon B-cell receptor engagement, allowing CD22 to regulate B-cell receptor signaling. CD22 participates in positive regulation through interactions with Src family tyrosine kinases and acts as an inhibitory receptor by recruiting cytoplasmic phosphatases via SH2 domains, which block signal transduction through dephosphorylation of signaling molecules. CD22's role in both positive and negative regulation of B-cell signaling, along with its specific expression pattern, makes it a valuable marker for antibody customers interested in B-cell-related research and diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell receptor CD22; B-lymphocyte cell adhesion molecule; B-lymphocyte cell adhesion molecule (BL-CAM); BL-CAM; CD22; CD22 antigen; FLJ22814; Lectin 2; Leu-14; MGC130020; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2 (Siglec-2); Siglec-2; T-cell surface antigen Leu-14
Gene Aliases: CD22; SIGLEC-2; SIGLEC2
UniProt ID: (Human) Q32M46
Entrez Gene ID: (Human) 933
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