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Invitrogen
Canine TNF-alpha Recombinant Protein
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Product Details
RCTNFAI
Product Specifications
Species
Dog
Expression System
P. pastoris
Amino acid sequence
Canine TNF-alpha, amino acids 77-233 (Accession # P51742)
Molecular weight
17.3 kDa
Class
Recombinant
Type
Protein
Activity
ED50 = 10 pg/mL; determined by the dose-dependent cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant canine TNFa was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D. The ED50 (or 1 unit of activity) for this effect in our laboratories is typically 10 pg/mL. Refs: Kitani, H. et al. (2002) Infect. Immun. 70:2210. Khabar, K.S. et al. (1995) Immunol. Lett. 46:107.
Expression: The mature, soluble form of canine TNF-alpha was expressed in Pichia pastoris, X-33. GenBank® Accession # P51742, amino acids 77 - 233. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VKSSSRTPSD.
Reconstitute with 0.5 mL of distilled water.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumour necrosis factor
Gene Aliases: cTNF; TNF; TNFA; TNFSF2; TNLG1F
UniProt ID: (Dog) P51742
Entrez Gene ID: (Dog) 403922
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