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Product Details
PEP-033
Product Specifications
Species Reactivity
Tag
Class
Synthetic
Type
Peptide
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
purified
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
PEP-033 is a 15 amino acid synthetic peptide whose sequence corresponds to N-terminal amino acid residues 26-39 of Green Fluorescent Protein . The sequence of this peptide is (amino to carboxy terminus): H26-K-F-S-V-S-G-E-G-E-G-D-A-T39- (C) (A C-terminal cysteine residue was added to facilitate coupling KLH).
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human prion protein, catalog # PA1-980. Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.
Reconstitute with 0.1 mL of distilled water.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
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