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Human ANG-1 Recombinant Protein, PeproTech®

Human ANG-1 Protein in Functional Assay (Functional)

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Human ANG-1 Protein (130-06-100UG) in Functional

Bioassay analysis of Human ANG-1 Recombinant Protein, PeproTech® (Product # 130-06-1MG).

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Product Details

130-06-100UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 8 publications 8 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

HeLa cells

Amino acid sequence

SNQRRSPENS GRRYNRIQHG QCAYTFILPE HDGNCRESTT DQYNTNALQR DAPHVEPDFS SQKLQHLEHV MENYTQWLQK LENYIVENMK SEMAQIQQNA VQNHTATMLE IGTSLLSQTA EQTRKLTDVE TQVLNQTSRL EIQLLENSLS TYKLEKQLLQ QTNEILKIHE KNSLLEHKIL EMEGKHKEEL DTLKEEKENL QGLVTRQTYI IQELEKQLNR ATTNNSVLQK QQLELMDTVH NLVNLCTKEG VLLKGGKREE EKPFRDCADV YQAGFNKSGI YTIYINNMPE PKKVFCNMDV NGGGWTVIQH REDGSLDFQR GWKEYKMGFG NPSGEYWLGN EFIFAITSQR QYMLRIELMD WEGNRAYSQY DRFHIGNEKQ NYRLYLKGHT GTAGKQSSLI LHGADFSTKD ADNDNCMCKC ALMLTGGWWF DACGPSNLNG MFYTAGQNHG KLNGIKWHYF KGPSYSLRST TMMIRPLDFH HHHHH

Tag

His-tag

Molecular weight

56.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

130-06-1MG will be provided as 10 x 100 µg (130-06-100UG).

130-06-500UG will be provided as 5 x 100 µg (130-06-100UG).

Recombinant Human ANG-1, derived from HeLa cells, is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0-70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein. The calculated molecular weight of Recombinant Human ANG-1 is 56.3 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Overfit deep neural network for predicting drug-target interactions.

iScience

Xiaolin X,Xiaozhi L,Guoping H,Hongwei L,Jinkuo G,Xiyun B,Zhen T,Xiaofang M,Yanxia L,Na X,Chunyan Z,Rui G,Kuan W,Cheng Z,Cuancuan W,Mingyong L,Xinping D

130-06 was used in In vitro experiments to identify fifteen compounds that interacted with TEK, a receptor tyrosine kinase contributing to vascular homeostasis, and the predicted AT9283 and dorsomorphin were experimentally demonstrated as inhibitors of TEK in human umbilical vein endothelial cells (HUVECs).

Fri Sep 15 00:00:00 EDT 2023

VEGF Induces Expression of Genes That Either Promote or Limit Relaxation of the Retinal Endothelial Barrier.

International journal of molecular sciences

McCann M,Li Y,Baccouche B,Kazlauskas A

130-06 was used in In vitro experiments to indicate that VEGF engages distinct sets of genes to induce and sustain barrier relaxation.

Wed Mar 29 00:00:00 EDT 2023

Functional microvascularization of human myocardium in vitro.

Cell reports methods

King O,Cruz-Moreira D,Sayed A,Kermani F,Kit-Anan W,Sunyovszki I,Wang BX,Downing B,Fourre J,Hachim D,Randi AM,Stevens MM,Rasponi M,Terracciano CM

130-06 was used in Cell Culture to study static and perfused models of human myocardial-microvascular interaction using a functionally vascularized in vitro model of human myocardium with widespread potential applications in basic and translational research.

Mon Sep 19 00:00:00 EDT 2022

Angiopoietin-1 Upregulates Cancer Cell Motility in Colorectal Cancer Liver Metastases through Actin-Related Protein 2/3.

Cancers

Rada M,Kapelanski-Lamoureux A,Tsamchoe M,Petrillo S,Lazaris A,Metrakos P

130-06 was used in In vitro experiments to suggest novel mechanisms by which Angiopoietin1 confers the development of vessel co-option in colorectal cancer liver metastases (CRCLMs), which, targeting this pathway, may serve as promising therapeutic targets to overcome the development of vessel co-option in CRCLM.

Sat May 21 00:00:00 EDT 2022

Angiopoietin-1 Upregulates Cancer Cell Motility in Colorectal Cancer Liver Metastases through Actin-Related Protein 2/3.

Cancers

Rada M,Kapelanski-Lamoureux A,Tsamchoe M,Petrillo S,Lazaris A,Metrakos P

Sat May 21 00:00:00 EDT 2022

Angiopoietins stimulate pancreatic islet development from stem cells.

Scientific reports

Karanth SS,Sun S,Bi H,Ye K,Jin S

Wed Jun 30 00:00:00 EDT 2021

Angiopoietin1 Deficiency in Hepatocytes Affects the Growth of Colorectal Cancer Liver Metastases (CRCLM).

Cancers

Ibrahim NS,Lazaris A,Rada M,Petrillo SK,Huck L,Hussain S,Ouladan S,Gao ZH,Gregorieff A,Essalmani R,Seidah NG,Metrakos P

130-06 was used in Sample Preparation to demonstrate that Ang 1 alone promotes the migration and growth of both human and mouse colon cancer cell lines These results provide evidence that high expression of Ang1 in the host liver is important to support vessel co-option (RHGP lesions) and when inhibited, favours the formation of angiogenic driven liver metastases (DHGP lesions).

Fri Dec 20 00:00:00 EST 2019

Angiopoietin1 Deficiency in Hepatocytes Affects the Growth of Colorectal Cancer Liver Metastases (CRCLM).

Cancers

Ibrahim NS,Lazaris A,Rada M,Petrillo SK,Huck L,Hussain S,Ouladan S,Gao ZH,Gregorieff A,Essalmani R,Seidah NG,Metrakos P

Fri Dec 20 00:00:00 EST 2019

Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation.

Proceedings of the National Academy of Sciences of the United States of America

Jeon JS,Bersini S,Gilardi M,Dubini G,Charest JL,Moretti M,Kamm RD

Tue Jan 06 00:00:00 EST 2015

Primary human acute myeloid leukaemia cells increase the proliferation of microvascular endothelial cells through the release of soluble mediators.

British journal of haematology

Hatfield K,Øyan AM,Ersvaer E,Kalland KH,Lassalle P,Gjertsen BT,Bruserud Ø

Thu Jan 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: ANG-1; Angiopoietin-1

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Gene Aliases: AGP1; AGPT; ANG1; ANGPT1; KIAA0003

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UniProt ID: (Human) Q15389

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Entrez Gene ID: (Human) 284

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Human ANG-1 Recombinant Protein, PeproTech®

Human ANG-1 Protein (130-06-100UG) in Functional

Product Details

130-06-100UG

Functional Assay (Functional)

In vitro Assay (IV)

Species

Published species

Expression System

Amino acid sequence

Tag

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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