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Human Amphiregulin Recombinant Protein, PeproTech®

Human Amphiregulin Protein in Functional Assay (FN)

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Human Amphiregulin Protein (100-55B-100UG) in FN

Bioassay analysis of Human Amphiregulin Recombinant Protein, PeproTech® (Product # 100-55B-1MG).

Product Image Gallery

Product Details

100-55B-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (FN)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 8 publications 8 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species

Human

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK

Molecular weight

11.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis.

Nature communications

Fankhaenel M,Hashemi FSG,Mourao L,Lucas E,Hosawi MM,Skipp P,Morin X,Scheele CLGJ,Elias S

100-55B was used in Cell Culture to establish Annexin A1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis.

Wed Jan 11 00:00:00 EST 2023

Schwann cell-derived amphiregulin enhances nerve regeneration via supporting the proliferation and migration of Schwann cells and the elongation of axons.

Journal of neurochemistry

Chen S,Chen Q,Zhang X,Shen Y,Shi X,Dai X,Yi S

100-55B was used in In vivo experiments to identify AREG as an important neurotrophic factor and thus provide a promising therapeutic avenue towards peripheral nerve injury.

Tue Aug 01 00:00:00 EDT 2023

GPR174 knockdown enhances blood flow recovery in hindlimb ischemia mice model by upregulating AREG expression.

Nature communications

Liu J,Pan L,Hong W,Chen S,Bai P,Luo W,Sun X,He F,Jia X,Cai J,Chen Y,Hu K,Song Z,Ge J,Sun A

100-55B was used in In vitro experiments to demonstrate that GPR174 negatively regulates angiogenesis and vascular remodeling in response to ischemic injury and that GPR174 may be a potential molecular target for therapeutic interventions of ischemic vascular diseases.

Tue Dec 06 00:00:00 EST 2022

GPR174 knockdown enhances blood flow recovery in hindlimb ischemia mice model by upregulating AREG expression.

Nature communications

Liu J,Pan L,Hong W,Chen S,Bai P,Luo W,Sun X,He F,Jia X,Cai J,Chen Y,Hu K,Song Z,Ge J,Sun A

Tue Dec 06 00:00:00 EST 2022

Interactions between cancer cells and immune cells drive transitions to mesenchymal-like states in glioblastoma.

Cancer cell

Hara T,Chanoch-Myers R,Mathewson ND,Myskiw C,Atta L,Bussema L,Eichhorn SW,Greenwald AC,Kinker GS,Rodman C,Gonzalez Castro LN,Wakimoto H,Rozenblatt-Rosen O,Zhuang X,Fan J,Hunter T,Verma IM,Wucherpfennig KW,Regev A,Suvà ML,Tirosh I

Mon Jun 14 00:00:00 EDT 2021

Targeting autocrine amphiregulin robustly and reproducibly inhibits ovarian cancer in a syngeneic model: roles for wildtype p53.

Oncogene

Lindzen M,Ghosh S,Noronha A,Drago D,Nataraj NB,Leitner O,Carvalho S,Zmora E,Sapoznik S,Shany KB,Levanon K,Aderka D,Ramírez BS,Dahlhoff M,McNeish I,Yarden Y

Sat May 01 00:00:00 EDT 2021

Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion.

iScience

Zhang Y,Guo A,Lyu C,Bi R,Wu Z,Li W,Zhao P,Niu Y,Na J,Xi JJ,Du Y

100-55B was used in Cell Culture to optimize the in vitro liver fibrotic niches and subsequently harvest their bioactive ingredients as niche extracts (NEs) so that pathology-relevant and tissue-specific NEs provide innovative and generalizable strategies for the discovery of optimal cellular niche and bioactive niche compositions.

Fri Nov 19 00:00:00 EST 2021

PD-L1 recruits phospholipase C and enhances tumorigenicity of lung tumors harboring mutant forms of EGFR.

Cell reports

Ghosh S,Nataraj NB,Noronha A,Patkar S,Sekar A,Mukherjee S,Winograd-Katz S,Kramarski L,Verma A,Lindzen M,Garcia DD,Green J,Eisenberg G,Gil-Henn H,Basu A,Lender Y,Weiss S,Oren M,Lotem M,Geiger B,Ruppin E,Yarden Y

Tue May 25 00:00:00 EDT 2021

Regulation of the EGFR/ErbB signalling by clathrin in response to various ligands in hepatocellular carcinoma cell lines.

Journal of cellular and molecular medicine

Liu Y,Calmel C,Desbois-Mouthon C,Sobczak-Thépot J,Karaiskou A,Praz F

Wed Jul 01 00:00:00 EDT 2020

Oncogenic mutant RAS signaling activity is rescaled by the ERK/MAPK pathway.

Molecular systems biology

Gillies TE,Pargett M,Silva JM,Teragawa CK,McCormick F,Albeck JG

Thu Oct 01 00:00:00 EDT 2020

Transient commensal clonal interactions can drive tumor metastasis.

Nature communications

Naffar-Abu Amara S,Kuiken HJ,Selfors LM,Butler T,Leung ML,Leung CT,Kuhn EP,Kolarova T,Hage C,Ganesh K,Panayiotou R,Foster R,Rueda BR,Aktipis A,Spellman P,Ince TA,Xiu J,Oberley M,Gatalica Z,Navin N,Mills GB,Bronson RT,Brugge JS

Mon Nov 16 00:00:00 EST 2020

Regulatory T-cells regulate neonatal heart regeneration by potentiating cardiomyocyte proliferation in a paracrine manner.

Theranostics

Li J,Yang KY,Tam RCY,Chan VW,Lan HY,Hori S,Zhou B,Lui KO

100-55B was used in Cell Culture to investigate the role of Treg in neonatal heart regeneration in mice.

Fri Aug 14 00:00:00 EDT 2020

Image-Based Profiling Can Discriminate the Effects of Inhibitors on Signaling Pathways under Differential Ligand Stimulation.

SLAS discovery : advancing life sciences R & D

Tanabe K,Inagaki A,Henmi Y,Satake M

100-55B was used in Cell Culture to investigate different cellular stimulation effects on efficacy of image-based compound profiling.

Sun Apr 01 00:00:00 EDT 2018

ZBTB20 regulates EGFR expression and hepatocyte proliferation in mouse liver regeneration.

Cell death & disease

Zhang H,Shi JH,Jiang H,Wang K,Lu JY,Jiang X,Ma X,Chen YX,Ren AJ,Zheng J,Xie Z,Guo S,Xu X,Zhang WJ

Tue May 01 00:00:00 EDT 2018

Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling.

Oncogene

Junk DJ,Bryson BL,Smigiel JM,Parameswaran N,Bartel CA,Jackson MW

Thu Jul 13 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Amphiregulin; amphiregulin B; AR; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor

Human Amphiregulin Recombinant Protein, PeproTech®

Human Amphiregulin Protein (100-55B-100UG) in FN

Product Details

100-55B-100UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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